Nogo受体对糖尿病大鼠视网膜神经节细胞凋亡的影响  

The Role of Nogo Receptor on the Apoptosis of Retinal Ganglion Cells in Diabetic Rats

作　　者：朱德淼[1] 刘学政[2] 

机构地区：[1]辽宁中医药大学中西医结合基础学科, 100032 [2] 辽宁医学院人体解剖学教研室

出　　处：《天津医药》2014年第3期217-219,I0005,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:31140072);辽宁省科技厅资助项目(项目编号:2011225015)

摘　　要：目的探讨Nogo受体对糖尿病大鼠视网膜神经节细胞(RGC)凋亡的影响及机制。方法 SD大鼠腹腔注射链脲佐菌素诱导30只糖尿病模型。分为对照组、糖尿病组、siNgR组、siRNA空白组,每组10只。siNgR组糖尿病大鼠玻璃体内注射Nogo受体反义核苷酸序列;siRNA空白组糖尿病大鼠玻璃体内注射阴性核苷酸序列。1个月后,免疫荧光组化检测Nogo受体与RGC标志物Brn3a的共存;以TUNEL染色观察RGC凋亡;试剂盒检测视网膜丙二醛(MDA)含量;Western blot检测视网膜Nogo受体及半胱氨酸天冬氨酸蛋白酶3(caspase-3)表达。结果 Nogo受体大量表达于视网膜RGC内,对照组、糖尿病组、siRNA空白组及siNgR组MDA含量分别为(3.68±0.47)、(8.07±1.24)、(7.54±1.53)及(5.12±0.62)μmol/g蛋白,RGC凋亡率分别为(5.1±0.2)%、(49.3±2.7)%、(45.6±1.8)%及(12.4±0.6)%,Nogo受体相对表达量分别为(0.18±0.07)%、(0.45±0.12)%、(0.40±0.09)%及(0.16±0.09)%,caspase-3相对表达量分别为(0.16±0.05)%、(0.40±0.18)%、(0.42±0.12)%及(0.17±0.08)%。与对照组相比,糖尿病组及siRNA空白组视网膜RGC凋亡明显,Nogo受体及caspase-3表达上调,MDA含量增加(P<0.05)。与糖尿病组相比siNgR组视网膜RGC凋亡减少、Nogo受体及caspase-3表达下调、MDA含量降低(P<0.05)。结论 Nogo受体表达上调参与了糖尿病视网膜RGC凋亡,其机制可能与Nogo受体上调caspase-3表达、诱发视网膜氧化应激有关。Objective To investigate the effect of Nogo receptor on the apoptosis of retinal ganglion cell （RGC） in di-abetic rats, and the potential mechanism thereof. Methods Thirty diabetic model rats were induced by intraperitoneal ad-ministration of streptozotocin. Model rats were randomly divided into control group, diabetes mellitus （DM） group, siNgR group and siRNA control group （n=10 for each group）. Diabetic rats in siNgR group were intravitreally administrated with No-go receptor antisense nucleotide. Diabetic rats in siRNA control group were intravitreally administrated with negative nucleo-tide. One month after diabetes onset, colocalization of Nogo receptor and Brn3a （marker of RGC） was observed by immunohis-tochemistry. The apoptosis of RGC was detected by TUNEL staining. The level of retinal malondialdehyde （MDA） was ob-served with kit, and the expressions of Nogo receptor and caspase-3 were detected with Western blot assay. Results It was found that the Nogo receptor was highly expressed in RGC. The levels of retinal MDA were （3.68＆#177;0.47）, （8.07＆#177;1.24）, （7.54＆#177; 1.53） and （5.12 ＆#177; 0.62） μmol/g protein for control group, DM group, siRNA control group and siNgR group. The apoptotic rates of RGC were （5.1 ＆#177; 0.2）%, （49.3 ＆#177; 2.7）%, （45.6 ＆#177; 1.8）%and （12.4 ＆#177; 0.6）%respectively. The expressions of Nogo receptor were （0.18 ＆#177; 0.07）%, （0.45 ＆#177; 0.12）%, （0.40 ＆#177; 0.09）%and （0.16 ＆#177; 0.09）%. The expressions of caspase-3 were （0.16 ＆#177; 0.05）%, （0.40＆#177;0.18）%, （0.42＆#177;0.12）%and （0.17＆#177;0.08）%. Compared with control group, there was significant increase in apoptosis of RGC, significantly up-regulated expressions in Nogo receptor and caspase-3, and significantly increased level of MDA in DM group and siRNA control group（P＆lt;0.05）. Compared with DM group, there were decreased apoptotic rate of RGC, de-creased expressions of Nogo receptor and casp

关 键 词：糖尿病 视网膜神经节细胞 RNA 小分子干扰 细胞凋亡 丙二醛 半胱氨酸天冬氨酸蛋白酶3 疾病模型 动物 NOGO受体 CASPASE 3 

分 类 号：R741.02[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]