产脂肪酶菌株的筛选、鉴定与产酶条件优化  

The Screening and Identification of a Lipase-Producing Strain and Its Culture Condition Optimization

作　　者：黎小军[1] 谢莲萍[1] 刘建宏[1] 朱婷婷[1] 刘蓉[1] 

机构地区：[1]新余学院医学与生命科学学院,江西新余338000

出　　处：《江西师范大学学报（自然科学版）》2014年第1期14-18,共5页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：江西省青年科学基金(20122BAB214011)资助项目

摘　　要：以橄榄油为唯一碳源,采用平板变色圈法,从土壤中筛选到1株脂肪酶高产菌XYU-6,经生理生化试验鉴定及16S rDNA序列分析,XYU-6被鉴定为洋葱假单胞菌(Burkholderia cepacia).设计单因素试验,对其产酶条件进行优化,优化后的培养条件为:葡萄糖1.00%,蛋白胨2.00%,豆油1.00%,KH2PO40.35%,K2HPO40.15%,MgSO40.05%,初始pH值为7.0,培养温度30℃,培养48 h,酶活可达24.1 U·mL-1,是优化前的1.65倍.A lipase producing strain XYU-6 was isolated from the soil by the method of flat discolor circle with olive oil as sole carbon source. XYU-6 was identified as Burkholderia cepacia by 16S rDNA sequencing and its biochemi- cal and physiological characteristics. The optimal conditions, glucose 1. 00%, peptone 2. 00%, soya-bean oil 1.00% ,KH2PO4 0.35% ,K2HPO4 0.15% and MgSO4 0.05% were obtained through one-factor experimental de- sign. Furthermore, the highest lipase activity 24.1 U mL-lwas achieved under the optimum conditions with initial pH 7.0 ,culture temperature 30 ℃ and culture time 48 h. These results indicated that the lipase activity was im- proved 1.65 fold compared to the original conditions.

关 键 词：脂肪酶 洋葱假单胞菌 筛选 鉴定 优化 

分 类 号：Q939[生物学—微生物学] TQ925[轻工技术与工程—发酵工程]