外源肉碱对鲤鱼背肌脂肪酸组成及其CPTI基因表达的影响  

Effects of exogenous carnitine on fatty acid composition and CPT I gene expression in muscle of common carp (Cyprinus carpio)

作　　者：董婧[1,2] 李林[2] 于业辉[2] 董晓庆[1] 陈玉珂[1] 裴怀全[1] 王秋举[1] 张东鸣[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]沈阳农业大学畜牧兽医学院,辽宁沈阳110866

出　　处：《西北农林科技大学学报（自然科学版）》2014年第1期29-36,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30671621;31302149);沈阳农业大学青年教师科研基金项目(20111015);吉林省发改委产业技术研究与开发项目;东北老工业基地外贸发展项目

摘　　要：【目的】研究外源肉碱对鲤鱼背肌脂肪酸组成及其CPT I基因表达的影响,为阐明肉碱调控脂肪酸代谢的机理提供理论依据。【方法】在基础饲料中分别添加0,50,100,150,200和400mg/kg L-肉碱,进行饲养试验,60d后取鲤鱼(Cyprinus carpio)背肌样品,测定其脂肪酸组成;提取鲤鱼背肌总RNA,采用同源克隆方法扩增CPT I基因的部分序列,Real-time PCR方法测定CPT I基因mRNA表达水平的变化。【结果】外源肉碱对鲤鱼背肌脂肪酸组成有显著影响(P<0.05),随饲料中L-肉碱添加量的增加,鲤鱼背肌∑SFA和∑MUFA脂肪酸含量显著升高(P<0.05),∑PUFA、EPA、DHA、∑n-3和∑n-6脂肪酸含量显著降低(P<0.05);同源克隆扩增获得了鲤鱼CPT I基因的部分cDNA序列(GenBank注册号为JQ361077),长度为857bp,同源性分析表明其与斑马鱼(Danio rerio)的同源性为89%。鲤鱼饲料中L-肉碱添加量为150mg/kg时,CPT I基因mRNA的表达丰度最高,为对照组的4倍。【结论】本试验条件下,外源肉碱添加量为150mg/kg时,显著影响鲤鱼幼鱼背肌脂肪酸组成和提高CPTI基因mRNA表达水平。[Objective] Effects of exogenous carnitine on fatty acid composition and CPT I gene expres- sion in muscle of common carp （Cyprinus carpio） were studied to better clarify the regulatory mechanism of carnitine on fatty acid metabolism. [Method] Six carp groups were fed with basal diet containing L- carnitine concentrations of 0,50,100,150,200, and 400 mg/kg for sixty days, and muscle samples were ex- tracted for detecting fatty acid composition and RNA were extracted for CPT I gene expression measure- ment using Real-time PCR. [Result] Fatty acid composition was significantly affected （P〈0.05） by L- carnitine. ∑SFA and ∑MUFA contents of carps fed with L-carnitine were significantly （P〈0.05） higher than those of the control group, while ∑PUFA, EPA, DHA, ∑n-3 and ∑n-6 contents were significantly （P〈0.05） lower. Partial cDNA of CPT I gene was cloned （GenBank accession number JQ361077） by ho- rnologous cloning, and the homologous alignment was analyzed. The obtained cDNA sequence was 857 bp in length,and its homology with zebra fish （Danio rerio） was 89%. 150 mg/kg L-carnitine resulted in the highest mRNA expression level, which was three times higher than that of the control group. [ConclusionI Adding 150 mg/kg L-carnitine to the basal diet significantly affected the fatty acid composition and CPT I gene expression level in muscle of common carp.

关 键 词：外源肉碱 鲤鱼 脂肪酸组成 CPT I基因表达 

分 类 号：S963.73[农业科学—水产养殖]