波叶山蚂蝗α-葡萄糖苷酶抑制活性研究  

α-Glucosidase Inhibitory Activity of Extracts from Desmodium sequax

作　　者：张龙[1,2] 王俊霞[3] 顾雪竹[4] 张前军[1] 

机构地区：[1]贵州大学化学与化工学院,贵阳550025 [2]贵州省产品质量监督检验院,贵阳550002 [3]河南大学中药研究所,河南开封475004 [4]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2014年第7期70-72,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药现代化项目(黔科合中药字[2010]5029);教育部春晖计划项目(Z2011036);贵阳市科技计划项目(2012204)

摘　　要：目的:评价波叶山蚂蝗提取物对α-葡萄糖苷酶的抑制活性。方法:通过建立体外α-葡萄糖苷酶抑制模型,对波叶山蚂蝗石油醚、乙酸乙酯、正丁醇提取物进行活性筛选。结果:波叶山蚂蝗3个提取物均有较好的α-葡萄糖酶抑制作用,抑制活性均远远大于阳性对照阿卡波糖(IC50=1 103.01 mg·L-1),其中以石油醚部位(DSPE)活性最好(IC50=45.16 mg·L-1)。乙酸乙酯部位(DSEA)和正丁醇部位(DSME)对α-葡萄糖苷酶抑制活性呈显浓度依赖性。结论:波叶山蚂蝗3个提取部位有较显著的α-葡萄糖苷酶抑制活性,具有开发应用的价值。Objective： To evaluate α-glucosidase inhibitory activity of extracts from Desmodium sequax. Method： By establishing α-glucosidase inhibitory model in vitro, the activity of petroleum ether extract, ethyl acetate extract and methanol extract from D. sequax was screened. Result： Three extracts from D. sequax showed good inhibitory activity, which showed far greater activity than that of acarbose （IC50=1 103.01 mg·L^-1）. Petroleum ether extract （DSPE） had the highest inhibitor activity （IC50=45.16 mg·L^-1）. α-glucosidase inhibitory activity of ethyl acetate extract （DSEA） and methanol extract （DCME） had concentration-dependent. Conclusion： Three extracts had more significant α-glucosidase inhibitory activity and great development and application value.

关 键 词：波叶山蚂蝗 Α-葡萄糖苷酶 抑制活性 

分 类 号：R284.1[医药卫生—中药学]