ATR-FTIR检测毕赤酵母发酵中甘油和甲醇浓度  

Measurement of glycerol and methanol concentrations in Pichia pastoris fermentations using ATR-FTIR

作　　者：吴胜[1] 杭海峰[1] 郭美锦[1] 储炬[1] 庄英萍[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《食品与发酵工业》2014年第3期1-7,共7页Food and Fermentation Industries

基　　金：国家973计划(2013CB733600);国家科技支撑计划(2011BAF02B02);中央高校基本科研业务费资助(WF1114019)

摘　　要：采用无机盐培养基毕赤酵母发酵生产重组人溶菌酶,以人工配置的标准溶液作为建模集,利用ATRFTIR结合PLS建立甘油和甲醇的预测模型,模型具有良好的稳定性和预测性,甘油预测模型的SEC和SEV分别为0.171和0.532 g/L,甲醇预测模型的SEC和SEV分别为0.129和0.248 g/L。在5 L发酵罐中运用建立的模型预测发酵液中甘油和甲醇的浓度,预测标准误差(SEP)分别为1.09和0.86 g/L,表明模型稳定,能有效指导实际生产。比较5 L发酵罐中分别采用FTIR调控和溶氧(DO-stat)调控甲醇浓度下的比生长速率(μ)和比生产速率(Qp),结果表明,FTIR调控下比生长速率和比生产速率均高于溶氧(DO-stat)调控。Mid-infrared FTIR spectroscopy is an efficient tool for the monitoring of bioprocesses. The use of Fourier transform mid-infrared spectroscopy （FTIR） combined with PLS to predict the concentrations of glycerol and methanol in fed-batch cultivations of Pichia pastoris in a chemical medium was investigated by using the synthetic solutions as calibration set. Generally, all models were robust and performed well, the calibration model allowed stand-rd errors of calibration （SEC） and standard errors of validation （SEV） for glycerol and methanol concentrations of 0. 171, 0. 532, 0. 129 and 0. 248 g/L, respectively. The results from 5 L Fed-batch experiments showed that FTIR data had a good agreement with control data, with standard error of prediction （SEP） of 1.09 and O. 86 g/L, respectively. Specific growth rate and specific production rate were compared between FTIR and DO-star to control methanol concentration. Results showed that FTIR could limit the methanol concentration under 2% , which could maintain a higher specific growth rate and specific production rate that was effective to enhance the production.

关 键 词：ATR-FTIR 毕赤酵母甘油 甲醇 比生长速率 比生产速率 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]