葎草组分测定、有效成分提取及不同提取物抗菌性的研究  

Study on Humulus scandens component determination, the effective component extraction and the antibacterial of different extract

作　　者：张勇[1] 黄进[1] 蔡凤英[1] 刘来亭[1] 胡新旭[2] 计成[3] 马秋刚[3] 

机构地区：[1]河南工业大学生物工程学院,河南郑州450001 [2]湖南省微生物研究所,湖南长沙410009 [3]中国农业大学动物科技学院,北京100193

出　　处：《饲料工业》2014年第5期52-56,共5页Feed Industry

摘　　要：文章研究葎草组分、有效成分提取及不同提取物的抗菌性。选用刚收获的葎草,经清洗、风干,测定葎草常规成分,采用95%乙醇(V/V)、三氯甲烷、乙酸乙酯、丙酮及蒸馏水作为提取剂提取葎草,采用滤纸片法和平板稀释法测定提取液抑菌效力。结果表明:葎草粗灰分、粗蛋白质、粗纤维、粗脂肪、无氮浸出物分别为8.3%、15.5%、19.1%、4.2%、47.5%。不同的提取剂得到葎草提取液固形物量以95%乙醇最高,为8.4 mg;不同的提取液对供试菌肠型点状产气单胞菌(Aeromonas punctata fintertinalis)、苏伯利产气单胞菌(Aeromonas sobria)和荧光假单胞菌(Pseudomonas fluoorescens)均有抑制作用。葎草具有一定的药用和营养价值,为葎草在动物饲料中的应用提供理论基础。The experiment study Humulus scandens component, the effective componenl extraction and the antibacterial of differenl extracts. Harvested Humulus scandens, clean, dried and determinated con- venlional components. Extracted Humt, lus scandens using 95% ethanol （V/V）, chloroform, ethyl ace- late, acetone and distilled water, delerminated antibacterial effect using filter paper method and agar dilution method. The results showed that the crude ash, crude protein, crude fiber, ether extract and ni- trngen-free extract from Humulus seandens were 8.3%, 15.5%, 19.1%, 4.2%, 47.5%, respectively. Compared with the ef[ective dry matters, HSE with 95% ethanol was the highest of all extract solve, nts and was 8.4 rag. Hunmlus scandens had inhibiting effect on Aeromonas punclata fintertinalis, Aermo- nas sobria and Pseudomonas fluoorescens.Hunulus scandens has some medicinal and nutritional value.

关 键 词：葎草 提取物 抑菌性 

分 类 号：S816.15[农业科学—饲料科学]