高黎贡山1株土壤淀粉酶产生菌产酶条件的优化  

Optimization on Enzyme Producing Conditions of Amylase-producing Strain in Gaoligong Mountain

作　　者：余丽[1] 晏爱芬[1] 

机构地区：[1]保山学院资源环境学院,云南保山678000

出　　处：《贵州农业科学》2014年第2期103-105,共3页Guizhou Agricultural Sciences

基　　金：云南省"十二五"省级学科保山学院生物学重点建设学科项目(09B002K)

摘　　要：为弄清高黎贡山1株土壤淀粉酶的最佳产酶条件，采用摇瓶培养、发酵液作为粗酶液进行酶活测定，研究了不同培养基（酸碱）、不同温度和培养时间对淀粉酶产生菌产酶活性的影响。结果表明：碱性条件培养3 d、30℃下吸光值最大，即产酶最多；酸性条件培养4 d、50℃下吸光值最大，即产酶最多。该淀粉酶产生菌在碱性条件下可获最高酶活，达411．81 IU/（mL＆#183;min），而在酸性条件下可保持酶活稳定，为301．31～368．18 IU/（mL＆#183;min）。To explore the optimum conditions of amylase-producing strain in Gaoligong Mountain,the effects of different culture medium and different temperature and time on the production of amylase were studied by shake flask culture,with fermentation broth as a crude enzyme solution.Results：Under alkaline condition,incubation time of three days,30℃,the absorbance reached the highest,indicating the producing amount was the highest; and the absorbance reached the highest under acidic condition incubation time four days,50℃.The highest enzyme activity,411.81 IU/（mL＆#183;min）,could be obtained under alkaline condition.Whereas the enzyme activity was stable under acidic condition,301.31~368.18 IU/（mL＆#183;min）.

关 键 词：淀粉酶产生菌 酶活力 产酶条件 高黎贡山 云南 

分 类 号：S182[农业科学—农业基础科学] X172[环境科学与工程—环境科学]