生物活性肽的抗炎功能及其对氧化应激的调节作用  

Anti-inflammation effect and regulatory role on oxidative stress of a bioactive peptide

作　　者：周婕慧[1] 金赢凯[1] 徐海红[1] 高扬[1] 林文珍 梁世排 张振江[1] 张少辉[1,3] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]浙江熊猫乳品有限公司,浙江温州325800 [3]上海交通大学陆伯勋食品安全研究中心,上海200240

出　　处：《中国乳品工业》2014年第3期4-6,14,共4页China Dairy Industry

摘　　要：通过动物实验研究乳源性生物活性肽Gln-Glu-Pro-Val-Leu(QEPVL)的抗炎能力,将32只小鼠随机分成空白组、模型组、低剂量组和高剂量组4组,利用脂多糖(Lipopolysaccharides,LPS)建立急性炎症模型,测定血清一氧化氮(NO)浓度和总抗氧化能力(TAOC)及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、过氧化氢酶(CAT)的浓度。结果表明,在100 mg/kg剂量下灌喂QEPVL水溶液30天可以显著降低血清NO浓度,并显著提高动物体内各种还原酶的酶活,提高机体抗氧化能力,缓解炎症引发的氧化应激状态,从而起到抗炎的功效。随着QEPVL剂量的加大,其抗炎效果加强。In this study, we investigated the anti-inflammation capacity of a milk-derived peptide Gln-Glu-Pro-Val-Leu （QEPVL） in mouse models. 32 Balb/c mouse were randomly separated into 4 groups： control group, model group, low-peptide group and high-peptide group. The LPS-induced inflammation model was built, nitric oxide （NO） of serum, total antioxidant capacity （T-AOC）, superoxide dismu- tase （SOD）, Glutathion Peroxidase（GSH-PX）, malondialdehyde （MDA）, catalase （CAT） in livers were determined. Results showed that serum NO and liver MDA were significantly decreased by QEPVL at a concentration of 100 mg/kg for 30 days administration while SOD, GSH- PX, CAT and T-AOC were significantly increased, which proved that the antioxidant capacity of liver were raised to ease the oxidative stress in mouse body, therefore proved QEPVL to have an anti-inflammation effect. The anti-inflammation capacity was found to be im- proved with the increasing of dosage.

关 键 词：生物活性肽 抗炎 氧化应激 调节作用 

分 类 号：Q516[生物学—生物化学]