不同时段嗜酸乳杆菌干预对β-乳球蛋白过敏缓解的作用  

Effects of treatment with Lactobacillus acidophilus at different times on preventing the bovine β-lactoglobulin allergy

作　　者：郑全玲 李艾黎[1] 戚晓熙[1] 郜雪飞 季晓梅[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室食品学院.哈尔滨150030

出　　处：《中国乳品工业》2014年第3期22-25,38,共5页China Dairy Industry

基　　金：乳品科学教育部重点实验室开放基金资助项目(2012KLD-SOF-07)

摘　　要：目的:分析和比较嗜酸乳杆菌预防和治疗β-乳球蛋白过敏的作用和效果,为抗过敏性能益生菌的使用提供科学依据。方法:BALB/c小鼠随机分为空白组、β-乳球蛋白过敏组、嗜酸乳杆菌预防组和嗜酸乳杆菌治疗组。建模结束后,采用酶联免疫吸附法(ELISA)检测各组小鼠血清中IgE及Thl/Th2型细胞因子(IL-12、IFN-γ、IL-4)的含量;RT-PCR法检测脾脏中T-bet和GATA-3 mRNA的表达量。结果:嗜酸乳杆菌干预可有效缓解β-乳球蛋白过敏,与过敏组小鼠比较,其血清总IgE和特异性IgE水平明显降低(P<0.05),IFN-γ、IL-12含量及T-bet mRNA表达量显著增加,IL-4含量及GATA-3 mRNA表达显著降低(P<0.05)。特别是嗜酸乳杆菌预防组的抗过敏效果优于治疗组,且预防组调节IFN-γ/IL-4比值(代表Thl/Th2细胞平衡)的能力显著优于治疗组(P<0.05)。结论:嗜酸乳杆菌预防β-乳球蛋白过敏的效果优于其治疗效果。Objective： To analyze and compare the preventative or therapeutic effects of L.acidophilus on the β-lactoglobulin （β-ig） allergy. Methods： The rats were randomly divided into 4 groups： blank group, milk allergy group, Lactobacillus aeidophilus prevention and treatment group. The levels of IgE and Thl/Th2 type cytokine （IL-12, IFN-γ IL-4） in the serum were detected by ELISA. the expressions of T-bet and GATA-3 mRNA in the spleen were measured by quantitative RT-PCR . Results： Compared with the milk allergy group, mice treated with L. acidophilus presented a significant decrease in total IgE and β-lg-specific IgE production as well as lower levels of IL-4 and GATA-3 mRNA expression. In contrast, the serum levels of IFN-γ and IL-12 were significantly increased after oral administration of L.acidophilus （P〈0.05）. Especially, when Balb/c mice were orally treated with L.acidophilus before sensitization with/3-1g, the IFN-γ//IL-4 value which represents the Thl/Th2 cell balance was dramatically improved compared with the L.acidophilus treatment group （/}〈0.05） .Conclusion： The anti-allergy effect of L.acidophilus prevention group is better than L.acidophilus treatment group

关 键 词：Β-乳球蛋白 嗜酸乳杆菌 抗过敏 BALB C小鼠 特异性IgE 酶联免疫吸附法 RT-PCR法 治疗效果 

分 类 号：Q93-33[生物学—微生物学]