Stable silver nanoparticles–aptamer bioconjugates for cellular prion protein imaging  

作　　者：Lei Zhan Li Peng Cheng-Zhi Huang 

机构地区：[1]Education Ministry Key Laboratory on Luminescence and RealTime Analysis, College of Chemistry and Chemical Engineering,Southwest University [2]College of Life Sciences, Southwest University [3]College of Pharmaceutical Sciences, Southwest University

出　　处：《Chinese Science Bulletin》2014年第10期964-970,共7页

基　　金：supported by the National Basic Research Program of China(2011CB933600)

摘　　要：Combining the unique optical properties of metal nanoparticles and the specific recognition of aptamer,aptamer–nanoparticle conjugates have been extensively used in a wide range of applications,particularly multifunctional nanoparticles for cell detection and molecular imaging.Conventional conjugates prepared by chemisorption of monothiol-modified oligonucleotides onto nanoparticle surfaces suffer from a lack of stability when exposed to a variety of small molecules.If silver is used in place of gold,then this lack of stability is even more pronounced.In this study,we reported here the effective and facile strategy of preparing stable silver nanoparticle–aptamer conjugates by in situ generation of strong metal affinity capping ligands,dithiocarbamates modified anti-prion protein aptamer.The conjugates produced are stable and can withstand NaCl concentration at0.25 mol/L.Meanwhile,they could be applied in the cellular prion protein imaging successfully.Combining the unique optical properties ofmetal nanoparticles and the specific recognition of apt-amer, aptamer-nanoparticle conjugates have been exten-sively used in a wide range of applications, particularlymultifunctional nanoparticles for cell detection andmolecular imaging. Conventional conjugates prepared bychemisorption of monothiol-modified oligonucleotidesonto nanoparticle surfaces suffer from a lack of stabilitywhen exposed to a variety of small molecules. If silver isused in place of gold, then this lack of stability is evenmore pronounced. In this study, we reported here theeffective and facile strategy of preparing stable silvernanoparticle-aptamer conjugates by in situ generation ofstrong metal affinity capping ligands, dithiocarbamatesmodified anti-priori protein aptamer. The conjugates pro-duced are stable and can withstand NaC1 concentration at0.25 mol/L. Meanwhile, they could be applied in the cel-lular prion protein imaging successfully.

关 键 词：nanoparticles APTAMER DITHIOCARBAMATE Cellular PRION protein 

分 类 号：O629.7[理学—有机化学]