鲁米诺化学发光法测定食品中的亚硫酸盐  

Determination of Sulfite in Foods Based on Luminol Chemiluminescence

作　　者：王建清[1] 徐丽红[1] 张玉[1] 王伟[1] 胡祺[1] 

机构地区：[1]浙江省农业科学院农产品质量标准研究所,浙江杭州310021

出　　处：《食品科学技术学报》2014年第1期65-68,共4页Journal of Food Science and Technology

基　　金：浙江省科技厅重大科技专项(2009C12052);浙江省农业科学院重点实验室前瞻性项目(2009C32093)

摘　　要：提出了运用化学发光法测定食品中亚硫酸盐的新方法,对影响化学发光的诸因素进行了实验和探讨,得出较佳检测条件：方法的定量限为4.6 mg/kg,亚硫酸盐质量浓度在1.0～10.0 mg/L与发光强度增强值（△I）呈良好的线性关系,R2为0.993 4.对6类样品进行了测定并做加标回收试验,平均RSD 3.05％,平均回收率93.0％,可用于食品中亚硫酸盐的定量与定性检测.The chemiluminescence technology was selected to establish a new method for the determina- tion of sulfites in foods. Various factors affecting the chemiluminescence detection were evaluated and the optimum determination conditions were obtained in this study. The detection limit of the method was 4.6 mg/kg. In the range of 1.0 - 10.0 mg/L, the sulfite concentration was positively correlated with the luminous intensity value （△I） , and the correlation coefficient （R2 ） was 0. 993 4. Spike recovery tests of six different samples were evaluated. The average RSD was 3.05% and the average recovery was 93.0%. Chemiluminescence determination results indicated that the new method was a good candidate for the qualitative and quantitative analysis of sulfites in foods.

关 键 词：鲁米诺试剂 亚硫酸盐 化学发光 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]