难治性癫痫患者脑内HIF-1α与多药耐药基因1表达的相关性及其意义  

Expressions and Correlation of HIF-1α and Multidrug Resistance Gene 1 in Brain Tissues of Patients with Refractory Epilepsy and Its Significance

作　　者：王广新[1] 马衍辉[1] 周荣双 于磊[1] 伊长英[1] 任士锋[1] 

机构地区：[1]山东大学齐鲁儿童医院,山东济南250022 [2]山东省临清市中医院

出　　处：《中国医学创新》2014年第6期3-5,共3页Medical Innovation of China

基　　金：国家自然科学基金项目(81071042);济南市卫生科技计划项目(2009-50)

摘　　要：目的:探讨难治性癫痫患者脑内缺氧诱导因子-1α(HIF-1α)与多药耐药基因1(MDR1)的表达情况、相关性及其意义。方法:收集9例难治性癫痫组手术切除脑组织标本及7例对照组脑组织标本,采用免疫组织化学染色方法检测HIF-1α和MDR1的表达情况,并分析HIF-1α与MDR1表达阳性率的相关性。结果:HIF-1α在难治性癫痫组脑组织中的阳性表达率77.8%(7/9)明显高于对照组的14.3%(1/7);MDR1在难治性癫痫组脑组织中的阳性表达率66.7%(6/9)明显高于对照组的14.3%(1/7),比较差异均有统计学意义(P<0.05);难治性癫痫组脑组织中HIF-1α与MDR1的表达呈正相关(r=0.76,P<0.05)。结论:难治性癫痫患者脑内HIF-1α和MDR1表达升高,且两者呈正相关。HIF-1α可能通过调控MDR1基因表达而参与难治性癫痫的形成。Objective：To explore expressions and correlation of hypoxia induced factor 1α（HIF-1α） and multidrug resistance gene 1（MDR1） in the brain tissues of patients with refractory epilepsy.Method：Brain tissues were collected from 9 patients with refractory epilepsy and 7 cases of the control group.The expression of HIF-1α and MDR1 were examined by immunohistochemistry,and the correlation between the positive expression rates of HIF-1α and MDR1 were analyzed.Result：The positive expression rate of HIF-1α in refractory epilepsy group（77.8%） was higher significantly than that in control group（14.3%）,the positive expression rate of MDR1 in refractory epilepsy group（66.7%） was higher significantly than that in control group（14.3%）,the differences were statistically significant（P〈0.05）.The expression of HIF-1α was positively correlated with the expression of MDR1（r=0.76,P〈0.05）.Conclusion：HIF-1α and MDR1 are over expressed in the brain of refractory epilepsy,and there is a positive correlation.HIF-1α may be involved in the pathogenesis of refractory epilepsy by regulating the expression of MDR1 gene.

关 键 词：缺氧诱导因子1Α 多药耐药基因1 难治性癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]