乳腺癌原代细胞特异性多肽的筛选  

Screen peptides specifically bonding to breast cancer primary cells

作　　者：孔冕[1] 王红月[1] 刘志勇[1] 侯传强[1] 李宝江[1] 

机构地区：[1]泰山医学院附属泰山医院乳腺外科,山东泰安271000

出　　处：《泰山医学院学报》2013年第12期890-892,共3页Journal of Taishan Medical College

基　　金：广东省科技计划项目(2010B031600066)

摘　　要：目的利用噬菌体展示技术从噬菌体随机十二肽库中筛选出能够特异性结合乳腺癌原代细胞的噬菌体多肽。方法以乳腺正常细胞为减性筛选细胞、乳腺癌原代细胞为靶细胞,对从商业购买的噬菌体随机十二肽库进行筛选,选取富集后的单克隆噬菌体,ELISA鉴定阳性噬菌体的特异性及亲和力,扩增阳性噬菌体克隆并提取DNA,测序后进行比对。结果经过3轮筛选,噬菌体得到近100倍富集,随机挑选11株单克隆噬菌体,ELISA鉴定显示2号噬菌体对乳腺癌细胞具有较高特异性及亲和力,测序结果显示其表达的多肽序列为未知序列。结论利用噬菌体筛选技术成功筛选出能够特异性结合乳腺癌原代细胞的特异性多肽,为进一步合成多肽应用于靶向治疗乳腺癌奠定基础。Objective：To isolate peptides bonding to breast cancer primary cells applying phage display technology. Methods： A phage display random 12 peptide library bought from commercial institution was screened by breast cancer primary cells and normal breast cells. The specification and affinity of the positive clones were identified by ELISA. The DNA of the positive clones were extracted after amplification and contrasted. Results： The phage clones were gathered about 100 times after 3 round of screening and 11 clones were chosen for ELISA. The ELISA result showed the clone NO.2 had a high specificity bonding to breast cancer primary cells. The sequence detection result showed the DNA sequence was unknown. Conclusion： We isolated a phage peptide bonding to breast cancer primary cells by using phage display technology, laid the foundation of the further study of the targeted therapy of breast cancer.

关 键 词：乳腺癌 噬菌体展示 噬菌体随机十二肽库 多肽 ELISA 

分 类 号：R737.9[医药卫生—肿瘤]