自动电位滴定法和高效液相色谱法测定深颜色果蔬中维生素C浓度的比较  

Comparison of vitamin C concentrations in dark colour fruits and vegetables detected by automatic potentiometric titration and HPLC method

作　　者：甘振威[1] 武广恒[2] 刘国良[1] 张娅婕[1] 徐坤[1] 谢林[1] 甘露[3] 

机构地区：[1]吉林大学公共卫生学院预防医学实验教学中心,吉林长春130021 [2]吉林大学基础医学院生物化学与分子生物学实验中心,吉林长春130021 [3]辽宁中医药大学药学院药物制剂专业2010级

出　　处：《吉林大学学报（医学版）》2014年第2期441-445,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81102115);吉林大学2011年实验技术项目资助课题(2011)

摘　　要：目的：探讨应用自动电位滴定法和高效液相色谱（HPLC）法定量测定深颜色果蔬中维生素C（Vc）浓度的可行性，为少量或批量果蔬样品Vc浓度的测定提供方法依据。方法：选取市售的15种蔬菜和15种水果的可食部分，加入一定量的草酸做Vc保护剂进行匀浆、过滤、离心处理，取一定量的上清液分别对其中的Vc浓度进行自动电位滴定和HPLC分析，对于同一红柿椒样品，分别用2种分析法对样品的Vc浓度进行准确度和精确度的测定。结果：2种分析法检测30种果蔬中Vc的浓度差异无统计学意义（P〉0．05），电位法对红柿椒中Vc浓度测定的回收率为98．34％～103．98％，HPLC法对Vc浓度测定的回收率为99．66％～101．49％；电位法测定红柿椒中Vc的相对标准偏差（RSD）为1.77％～2．88％，HPLC法测定Vc的RSD为0．15％～0．32％，且HPLC法线性关系良好（R^2=0．9999）。结论：2种分析法对深颜色果蔬样品中Vc浓度测定的准确度和精密度均能满足定量分析的要求，均为较理想的测试方法。Objective To explore the feasibility of quantitatively measuring the concentrations of vitamin C （Vc） in dark colour fruits and vegetables detected by automatic potentiometric titration and high performance liquid chromatography （HPLC）, and to provide experimental basis for measurement of Vc concentrations in a few or amount of fruits and vegetables. Methods 15 kinds of fruits and 15 kinds of vegetables sold in supermarket were selected. After adding a small quantity of oxalic acid, these samples were crisped, filtered, and centrifuged sequentially, and then the concentrations of Vc in upper clean liquid were determined by automatic potentiometrie titration and HPLC method. Accuracy and precision test for the same sample were also done with two methods. Results There were no significant differences between the detection results Of Vc concentrations detected by two methods （P〉0. 05）, and the recovery rates in red pepper detected with automatic potentiometric titration and HPLC methods were 98.34% - 103.98% and 99.66%-101.49%, respectively; the relative standard deviation （RSD） were 1.77%-2.88% and 0.15%0-0.32%, respectively. The HPLC data had good linear relationship （R^2=0. 999 9）. Conclusion The two kinds of test methods are ideal methods for the determination of Vc concentrations in dark colour fruits and vegetables. They both have good accuracy and precision for the requirement of quantity analysis.

关 键 词：电位滴定 色谱法 高效液相 维生素C 果蔬 准确度 精确度 

分 类 号：R151.3[医药卫生—营养与食品卫生学] R331[医药卫生—公共卫生与预防医学]