固定化丁酸梭菌转化甘油生产1,3-丙二醇的研究  

Microbial Production of 1,3-Propanediol from Glycerol by Immobilized Clostridiumbutyricum

作　　者：张蓝蓝[1,2] 王世珍[1,2] 朱春杰[1,2] 方柏山[1,2,3] 

机构地区：[1]厦门大学化学化工学院,福建厦门361005 [2]厦门市合成生物技术重点实验室(厦门大学),福建厦门361005 [3]醇醚酯化工清洁生产国家工程实验室(厦门大学),福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2014年第2期227-232,共6页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(21076172);福建省高校产学合作科技重大项目(2010H6023)

摘　　要：通过比较棉纤维织物、聚乙烯醇缩甲醛柱体、活性炭颗粒在35℃、厌氧条件下吸附丁酸梭菌(Clostridium butyricum)将甘油转化为1,3-丙二醇的有效性,选择活性炭颗粒作为固定化丁酸梭菌的较佳载体.以活性炭颗粒作为吸附载体时,初始甘油质量浓度从20g/L提高至252g/L,最终1,3-丙二醇质量浓度从9.5g/L增加至38.3g/L,同时残余甘油质量浓度从0g/L提高至96.3g/L,1,3-丙二醇的时空产率从0.7g/(L·h)提高至2.2g/(L·h),摩尔得率从65%降低至58%.Clostridium butyricum was immobilized on the cotton fiber,polyvinyl alcohol column and activated carbon granule to produce 1,3-propanediol from glycerol.Fermentation in continuous culture was carried out in a fixed-bed bioreactor.Results showed that the activated carbon granule has desirable properties,such as biomass enrichment on the granule and operation convenience for continuous culture.When glycerol continuous loading rate raised from 0.24g/(L·h)to 3.00g/(L·h),the productivity of 1,3-propanediol raised from 0.7g/(L·h)to 2.2g/(L·h),the residual concentration of 1,3-propanediol raised from 9.5g/L to 38.3g/L,the yield of 1,3-propanediol reduced from 65%to 58%.

关 键 词：甘油 1 3-丙二醇 填充床 固定化细胞 发酵 

分 类 号：TQ92[轻工技术与工程—发酵工程]