4种竹子竹叶黄酮质量分数及抗氧化活性的季节变化  

Seasonal flavonoid and anti-oxidant changes in leaves of four bamboo species

作　　者：潘佳佳[1,2] 叶生月 丁东栋 金政[1] 杨萍[2] 

机构地区：[1]浙江农林大学林业与生物技术学院,浙江临安311300 [2]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300 [3]桐庐县林业技术推广中心站,浙江桐庐311500

出　　处：《浙江农林大学学报》2014年第2期280-284,共5页Journal of Zhejiang A&F University

基　　金：杭州市科技发展计划项目(20101032B58);国家级大学生创新创业训练计划项目(201210341006);浙江省大学生科技创新活动计划(新苗人才计划)项目(2012R412006)

摘　　要：以毛竹Phyllostachys edulis,雷竹Phyllostachys violascens,石竹Phyllostachys nuda和高节竹Phyllostachys prominens等4种竹子为研究对象,用硝酸铝-亚硝酸钠比色法测定黄酮质量分数,并采用DPPH·(1,1-二苯基-2-三硝基苯肼)法和ABTS+·[2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐]法测定竹叶黄酮的抗氧化活性,以考察竹叶黄酮质量分数和抗氧化活性随季节和竹种的变化规律。结果表明:春季毛竹的竹叶黄酮质量分数最高(2.12%±0.15%)(P<0.05),夏季、秋季和冬季4种竹子间无显著性差异(P>0.05);石竹的竹叶黄酮质量分数四季变化不显著(P>0.05),毛竹夏季的竹叶黄酮质量分数(2.36%±0.60%)高于冬季(1.52%±0.04%)(P<0.05),雷竹(2.40%±0.17%)和高节竹(2.07%±0.13%)的竹叶黄酮质量分数以夏季最高。4种竹子的竹叶黄酮对DPPH·的清除能力都表现在夏季较强,四季的各竹种竹叶黄酮对DPPH·的清除能力以毛竹较强。4种竹子的竹叶黄酮对ABTS+·的清除能力都表现在春季和秋季较强,四季各竹种间差异性不大(P>0.05)。Leaves of four bamboo species： Phyllostachys edulis, Phyllostachys violascens, Phyllostachys nuda, and Phyllostachys prominens were studied to determine variation in flavonoid content and anti-oxidant activity for different seasons. Aluminum nitrate-sodium nitrite colorimetry was used to find the flavonoid content with 2,2 diphenyl-l-picrylhydrazyl （DPPH＇） and 2,2-azino-bis-3-ethylbenzothiazoline-6- sulfonic acid （ABTS＋-） being employed to discover anti-oxidative activity. Results in spring showed that flavonoid content of Ph. edulis was highest （2.12%±0.15%） （P〈0.05）, but in summer, autumn, and winter, there were no significant differences among the four bamboo species （P〉0.05） in the test flavonoid content. Flavonoid content of Ph. nuda did not change significantly （P〉0.05） during the four seasons; for Ph. edulis, summer flavonoid content （2.36%± 0.6% ） was higher than winter （1.52% ±0.04% ） （P〈0.05）; and for Ph. violascens （2.40% ±0.17% ） and Ph. prominens （2.07%±0.13%） the highest flavonoid content was in summer. The scavenging ability of DPPH- for bamboo leaf flavonoids was higher in summer with Ph. edulis being best. The scavenging ability of ABTS＋. forbamboo leaf flavonoids was greater in spring and autumn with no significance （P〉0.05） between species. These results can be used for the exploitation of bamboo resources.

关 键 词：植物生理学 竹叶黄酮 DPPH·清除能力 ABTS+·清除能力 抗氧化活性 四季变化 

分 类 号：S795[农业科学—林木遗传育种] S718.3[农业科学—林学]