亮绿褪色光度法测定酵母中钴含量  

Determination of Cobalt Content in Yeast by Fading Spectrophotometry with Bright Green

作　　者：李人宇[1] 印圆圆 陶明涛[1] 

机构地区：[1]连云港师范高等专科学校,江苏连云港222006

出　　处：《中国调味品》2014年第4期112-115,共4页China Condiment

摘　　要：研究钴（Ⅱ）与硫氰酸钾和亮绿的络合反应，建立褪色光度法测定钴含量的新方法。通过试验优化酸度、硫氰酸钾用量、亮绿用量、反应温度和反应时间等测定条件。结果表明：最大褪色波长为624 nm，表观摩尔吸光系数为2．2＆#215;105 L/mol＆#183;cm，钴（Ⅱ）浓度在0～0．30μg/mL范围内服从比尔定律，检出限为2．5μg/L。方法用于测定酵母中钴含量，结果与原子吸收光谱法一致，相对标准偏差为0．53％～0．81％（n ＝5），回收率为98．1％～101．5％。Study the complexation reaction of Co （Ⅱ）-potassium thiocyanate-light green system,a new fading spectrophotometry for determination of cobalt content is established.The optimal determina-tion conditions are studied after optimization of acidity,dosage of potassium thiocyanate and bright green,reaction temperature and time by experiments.The results show that the maximum fading wavelength is at 624 nm,the apparent molar absorption coefficient is 2.2＆#215;105 L/mol＆#183;cm,the cobalt （II）content within the range of 0~0.30μg/mL obeys Beer＆#39;s law,the detection limit is 2.5μg/L. This method is applied for the determination of cobalt content in yeast.The results obtained by this method are consistent with those obtained by atomic absorption spectrometry.The RSD is in the range of 0.53%~0.81% （n= 5）,and the recovery is in the range of 98.1%~101.5%.

关 键 词：褪色光度法 钴 酵母 

分 类 号：TS201.3[轻工技术与工程—食品科学]