UFLC-MS-MS方法快速筛查葡萄酒和辣椒中的17种工业染料  

Rapid determination of 17 industrial dyes in grape wine and chilli powder by UFLC-MS-MS

作　　者：罗诗萌 于秋红[1] 牛晓梅[1] 张志荣[1] 张来颖[1] 赵建忠[1] 

机构地区：[1]北京市丰台区疾病预防控制中心,北京100071

出　　处：《中国卫生检验杂志》2014年第6期819-823,共5页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立超快速液相色谱电喷雾串联四级杆质谱(UFLC-MS-MS)快速筛查食品中17种工业染料的方法。方法用乙腈作提取液对样品中被测色素进行提取。提取液经C18柱梯度洗脱,流动相为甲醇和0.1%的甲酸水溶液。采用多反应监测(MRM)模式进行检测,17种工业染料均为正离子模式。采用基质匹配,外标法进行定量。结果 17种工业染料在红酒中的检出限为0.2μg/kg^9μg/kg,定量限为0.6μg/kg^30μg/kg、回收率为59.7%~115.3%、相对标准偏差为1.2%~14.7%,在辣椒粉中检出限为0.2μg/kg^9μg/kg、定量限0.6μg/kg^30μg/kg、回收率为70.4%~121.7%、相对标准偏差为2.3%~18.4%。结论该方法简便、快速,适合葡萄酒和辣椒粉中非法添加工业染料的快速筛查。Objective To establish a method for rapid screening of 17 industrial dyes in grape wine and chilli powder by ultra fast liquid chromatography and tandem mass spectrometry. Methods The pigments in the samples were extracted with acetoni- trile, and then separated on a C 18 column with methanol -0.1% formic acid solution as mobile phase for gradient elution. With multiple reaction monitoring （MRM） mode, the 17 industrial dyes were all detected in positive ion mode. The matrix matching and exteMal standard method were used for quantitation. Results In grape wine, the detection limits of the 17 industrial dyes were 0.2 μg/kg - 9 tLg/kg, the limits of quantitation were 0.6 μg/kg - 30 μg/kg, the rates of recovery were 59.7% - 115.3%, and the relative standard deviations were 1.2% - 14.7%. While in chilli powder, those were 0.2 ttg/kg -9 μg/kg, 0.6 μg/kg - 30μg/kg, 70.4% - 121.7% and 2.3% - 18.4% respectively. Conclusion The method is simple and conven- ient, rapid, easy and suitable for screening the illegal industrial dyes in grape wine and chilli powder.

关 键 词：工业染料 葡萄酒 辣椒粉 超快速液相色谱-串联质谱 

分 类 号：O657.63[理学—分析化学]