食品中食源性病毒快速检测方法的建立研究  

Research on the rapid detection method of food-borne viruses in food

作　　者：雷永良[1] 王晓光[1] 梅少林[1] 陈秀英[1] 叶碧峰[1] 

机构地区：[1]丽水市疾病预防控制中心,浙江丽水323000

出　　处：《中国卫生检验杂志》2014年第6期855-857,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省公益技术研究社会发展项目(2012C33002);丽水市公益性技术应用研究项目(2011JYZB30);丽水市医学重点建设学科科技项目(丽科(2011)58号)

摘　　要：目的建立诺如病毒、轮状病毒、甲肝病毒的分子快速检测方法,为食品中食源性病毒检测提供技术支持。方法建立3种食源性病毒RT-PCR检测方法,通过梯度稀释的阳性对照对检测条件进行优化,分析其特异性和准确性。同时对临床表现为水样便,伴发热、恶心、呕吐等排除细菌性胃肠炎的患者腹泻样本和与食源性病毒流行有关的食品标本进行方法验证和食源性病毒调查。结果优化试验验证了诺如病毒、轮状病毒、甲肝病毒各自的RT-PCR特异性;并通过在非细菌性胃肠炎患者腹泻标本中检出诺如病毒、轮状病毒验证了其准确性。结论建立了诺如病毒、轮状病毒、甲肝病毒的分子快速检测方法。本地区病毒性胃肠炎的主要病原体为诺如病毒和轮状病毒,且轮状病毒的感染率高于诺如病毒。Objective To establish a molecular method for rapid detection of norovirus, rotavirus and hepatitis A virus, and provide technical suppo.rt for food - borne virus detection. Methods Three kinds of PT - PCR methods were set up for food - borne virus detection, and the testing conditions were optimized through positive control, the specificity and accuracy of the method were analyzed too. At the same time, the diarrhea specimens of non - bacterial gastroenteritis patients and food samples related to food - borne virus were detected for method validation and food - borne virus investigation. Results Optimization test has proved the specificity of RT - PCR in detection of norovirus, rotavirus and hepatitis A virus, while the checkout of norovirus and rotavirus from the non - bacterial gastroenteritis patients has validated the accuracy of the method. Conclusion A rapid molecular detection method of rorovirus, rotavirus and hepatitis A virus was successfully established. The main pathogens of vi- ral gastroenteritis are norovirus and rotavirus in Lishui, and rotavirus infection rate is higher than norovirus.

关 键 词：食品 食源性病毒 检测 方法 

分 类 号：R155.34[医药卫生—营养与食品卫生学]