壳聚糖和洗必泰对牙龈卟啉菌的协同抗菌作用  

Synergistic antimicrobial effect of chitosan and chlorhexidine on Porphyromonas gingivalis

作　　者：薛丹 许哲[2] 孙江 冯延民[3] 孙俊毅[2] 

机构地区：[1]大连市口腔医院牙周科,116023 [2]西安交通大学口腔医院牙周科 [3]吉林大学口腔医学院生化教研室

出　　处：《现代口腔医学杂志》2014年第2期76-79,共4页Journal of Modern Stomatology

基　　金：国家自然科学基金-青年科学基金项目(81202729);陕西省自然科学基金(2012JM74012)

摘　　要：目的评估壳聚糖和洗必泰对牙龈卟啉菌的协同抗菌效果。方法将壳聚糖,洗必泰与复合树脂粉混匀制成含有壳聚糖浓度为0%,0.4%,0.8%,1.6%,洗必泰浓度为0.02%,直径为6mm标准大小药敏块。将接种了牙龈卟啉菌的培养基分别放置不同浓度的药敏块,37℃厌氧培养72小时。结果牙龈卟啉菌的培养基中,抑菌环直径的大小随着壳聚糖浓度的增加而扩大,并可看出载有洗必泰的壳聚糖药敏块的抑菌效果比单纯的壳聚糖的抑菌效果更好。结论壳聚糖具有抗牙龈卟啉菌的作用,壳聚糖和洗必泰具有协同抗菌作用,联合应用比单独使用壳聚糖能取得更好的菌斑控制效果。Objective To evaluate the effect of synergistic antimicrobial effect of chitosan and chlorhexidine on periodontal pathogens Porphyromonas gingivalis. Methods Mixed chitosan, chlorhexidine and composite resin powder together producing drug blocks. The concentration of chitosan was 0%, 0.4%, 0.8%, 1.6%, and chlorhexidine concentration was 0.02%. The diameter of drug blocks was 6mm, using ultraviolet to sterilize the blocks. Porphyromonas gingivalis Inoculated susceptibility block medium were placed at different concentrations for 72 hours, 37 ~C in anaerobic culture. Results The diameter of inhibition zone extended with increasing of the concentration of chitosan, and it can be seen that the effect of the chitosan block loaded chlorhexidine was better than the result of the pure chitosan block. Conclusion Chitosan has the antimicrobial effect on Porphyromonas gingivalis, The synergistic antimicrobial effect of the chitosan and chlorhexidine is satisfying.

关 键 词：壳聚糖 洗必泰 抑菌 牙龈卟啉菌 

分 类 号：R780.2[医药卫生—口腔医学]