产多不饱和脂肪酸真菌的菌株筛选与鉴定  

Screening and Identifying of Fungal Strains Producing Polyunsaturated Fatty Acids

作　　者：肖洋[1] 胡开成[2] 周礼红[2] 李祝[2] 陈青[3] 秦江辉[2] 郭康翔 

机构地区：[1]国家酒料及饮料质量监督检验中心 贵州省产品质量监督检验院,贵州贵阳550001 [2]贵州大学生命科学学院真菌资源研究所,贵州贵阳550025 [3]贵州大学化学与化工学院,贵州贵阳550025

出　　处：《山地农业生物学报》2014年第1期18-23,共6页Journal of Mountain Agriculture and Biology

基　　金：毕节地区马铃薯产业科技联合专项[毕署科(2010)04号]

摘　　要：经菌株分离培养,采用苏丹黑B染色法初筛、摇瓶法复筛的方法,从土壤中筛选得到16株具有较好合成油脂能力的真菌菌株。对16株菌株的产油量和脂肪酸组成分析表明,该16株菌株油脂组成中C18∶0、C18∶1和C18∶2含量都较高,其中菌株1.12、1.11、2.2、12.8、水3.33可以合成C20以上的脂肪酸。对产油脂菌株进行形态观察鉴定,确定为青霉属(Penicillium Link)、曲霉属(Aspergillus Micheliex Fr.)、拟青霉属(Paecilomyces Bainier)、梨孢帚霉属(Scopulariopsis Bainier)和枝孢属(Cladosprium Link)。The sixteen fungi strains having a good ability of producing oils were screened form soil samples by the methods of isolated culture, preliminary screening with Sudan Black B stain and further screening by shake- flask culture. The oil output and fatty acid composition analysis showed that the sixteen trains had strong ability of synthesizing C18 fatty acids. Strains of 1.12, 1.11, 2.2, 12.8 and Shui3.33 can produce the C20 above of fatty acids. The strains of producing oils were identified as Penicillium Link, Aspergillus Mieheliex Fr. , Paecilomyces Bainier, Scopulariopsis Bainier, Cladosprium Link.

关 键 词：真菌 多不饱和脂肪酸 筛选 鉴定 

分 类 号：Q939[生物学—微生物学]