共振光散射法检测猪肉盐酸克伦特罗  

Monitoring clenbuterol hydrochloride in pork by resonance light scattering

作　　者：黄明元[1] 曾晓玲[2] 翟璐[1] 余敏旋 

机构地区：[1]广东医学院公共卫生学院,广东东莞523808 [2]广东医学院2009级学生,广东东莞523808

出　　处：《现代预防医学》2014年第8期1472-1473,1477,共3页Modern Preventive Medicine

基　　金：2012年广东医学院大学生创新实验项目自然科学类课题(ZYDG004);2012年广东省大学生创新创业训练计划项目(1057112053);湛江市科技功关项目(2011C3107014)

摘　　要：目的 建立食品中盐酸克伦特罗残留量的共振散射光检测方法。方法 环糊精与盐酸克伦特罗形成包合物，用荧光分光光度计测定包合物的共振散射光强度，以强度差异值对包合物中盐酸克伦特罗含量进行检测。结果 在波长为358 nm处共振光强度差异与盐酸克伦特罗的浓度变化呈线性关系。当实验温度为60℃，pH＝5.0和静置时间为60 min时，方法的检出限为1.2 ng/ml，线性范围为0～10.0 ng/ml，回收率为88％～118％。结论 方法可靠、简便，可用于食品中盐酸克伦特罗的测定。Objective The aim of study was to establish the methodology for monitoring clenbuterol hydrochloride in pork using res- onance light scattering. Methods After beta cyclodextrin and clenbuterol hydrochloride were combined, the intensity of resonance scattering light spectrum was measured, and the intensity difference of resonance scattering light was used to quantify the level of clenbuterol hydrochloride. Resoits At the wavelength of 358 nm, the resonance light scattering intensity was proportional enhanced with increasing clenbuterol hydrochloride level. In the experiment condition where the pH was 5.0, temperature was 60~C and the equilibration time was 60 min, the detection limit was 1.2 ng/mL, with a linear range between 0 and 10.0 ng/mL, and a recovery rate between 88% and 118%. Conclusion This method is reliable and simple, and can be used for the determination of clenbuterol hy- drochloride level in pork.

关 键 词：盐酸克伦特罗 共振散射光 环糊精 猪肉 

分 类 号：R155[医药卫生—营养与食品卫生学]