双歧杆菌培养及冻干菌粉制备工艺研究  

Culture of Bifidobacterium and Its Freeze-drying Bacterial Powder Production Technology

作　　者：张帆[1] 胡铁锋[1] 郑李彬[1] 李红强[1] 张瑾[1] 

机构地区：[1]河北科技师范学院,河北秦皇岛066000

出　　处：《安徽农业科学》2013年第35期13752-13755,共4页Journal of Anhui Agricultural Sciences

基　　金：河北省杰出青年基金项目;河北省高等学校科学研究计划项目(QN20131019);秦皇岛市科学技术研究与发展计划项目(2012022A009);河北科技师范学院科研创新团队项目(CXTD2012-06)

摘　　要：[目的]优化双歧杆菌培养及冻干菌粉制备工艺。[方法]通过测定生长曲线、酸度曲线、冻干菌粉含水量,筛选冻干保护剂等研究双歧杆菌培养体系及真空冷冻干燥过程。[结果]试验得出,双歧杆菌在牛奶还原培养基中培养30 h,pH约为4.5时,发酵液活菌数含量最高。真空冷冻干燥过程中加入单一保护剂100 g/L葡萄糖处理后,活菌率达到35.01%;加入复合保护剂(甘油+葡萄糖+海藻糖)后,活菌率可达72.09%。双歧杆菌真空冷冻干燥后,菌粉含水率约为3%(质量分数),冻干效果良好,益于保藏。[结论]试验可为深入研究双歧杆菌活菌粉制剂提供理论依据。[ Objective ] To optimize culture of Bifidobacterium and production technology of its freeze-drying bacterial powder. [ Method] Test growth curve, acidity curve, water content of freeze-drying bacterial powder i and screen freeze-drying protective agent, to study culture system of Bifidobacterium and the process of its vacuum freeze drying. [ Result ] After being cultured for 30 h in milk reducing medium, pH 4.5, quantity of viable bacteria in zymotic fluid achieves the highest level. After adding 100 g/L glucose in the process of vacuum freeze drying, the rate of viable bacteria achieves 35.01% ; after adding compound protective agent （ glycerol ＋ glucose ＋ mycose）, the rate of viable bacteria achieves 72.09%. After vacuum freeze drying, water content of the bacterial powder is 3% （mass fraction）, so it is suitable for being pre- served. [ Conclusion] The study provides a theoretical basis of producing bacterial powder of Bifidobacterium.

关 键 词：双歧杆菌 真空冷冻干燥 冻干保护剂 

分 类 号：S188[农业科学—农业基础科学]