响应曲面法优化鲁梅克斯叶蛋白提取条件  

Optimize extraction condition of leaf protein from Rumex by response surface methodology

作　　者：靳志明[1,2] 冯大伟[1,2] 刘胜一[1,2] 杜鹃[1,2] 衣悦涛[1,2] 

机构地区：[1]中国科学院烟台海岸带研究所,烟台264003 [2]中国科学院大学,北京100049

出　　处：《食品科技》2014年第4期209-213,共5页Food Science and Technology

基　　金：国家"863"计划项目(2012AA021205);"十二五"国家科技支撑计划项目(2011BAC02B04);"泰山学者"建设工程专项

摘　　要：以叶蛋白提取率为指标,来优化鲁梅克斯叶蛋白提取工艺,对影响叶蛋白提取率较大的提取温度、液料比和提取时间3因素进行研究。在单因素实验的基础上,采用响应曲面法,得到最优提取工艺条件。最优提取条件为:提取温度51.84℃、液料比41.95、提取时间49.88min,此条件下鲁梅克斯叶蛋白提取率为36.34%,实际验证值为36.08%,与估计值较为吻合。The objective of this study was to optimize the extraction condition of leaf protein from Rumex. In order to obtain the high extraction ratio, extraction temperature, liquid-to-solid ratio and extraction time were considered in this experiment. On the basis of single factor experiment, response surface methodology was designed to optimize conditions. The optimum values of the three conditions for improved leaf protein extraction were determined as follows： extraction temperature 51.84 ℃, liquid- to-solid ratio 41.95, extraction time 49.88 min. Under the optimized conditions, the extraction ratio was determined to be 36.34%. The verification value was 36.08%, which was in good agreement with the estimate.

关 键 词：鲁梅克斯 叶蛋白 提取率 响应曲面法(RSM) 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]