异味物质β-环柠檬醛降解菌的分离和鉴定  

ISOLATION AND IDENTIFICATION OF β-CYCLOCITRAL DEGRADING BACTERIA

作　　者：代志刚[1,2] 蒋永光[1,2] 谷依露[1,2] 胡晗华[1] 李仁辉[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2014年第2期222-226,共5页Acta Hydrobiologica Sinica

基　　金：国家"十二五"水专项(2012ZX07101-02-001-01)资助

摘　　要：β-环柠檬醛(β-cyclocitral)是由微囊藻产生的主要藻源性异味污染物之一。利用稀释涂平板法,从采集到的微囊藻水华水样中分离得到两株降解β-环柠檬醛的菌株DH16和DH18,16S rRNA序列比对和系统进化树分析表明它们分别属于食酸菌属(Acidovorax)和不动杆菌属(Acinetobacter)。实验室保藏的微囊藻毒素降解菌Novosphingobium sp.THN1对β-环柠檬醛也有降解效果,该菌属于新鞘氨醇杆菌属(Novosphingobium)。对三株菌进行以β-环柠檬醛为唯一碳源的培养实验,气相色谱检测分析表明DH18和THN1两株菌具有高效降解β-环柠檬醛的能力,可以作为研究β-环柠檬醛生物降解机理的理想材料。β-cyclocitral, one of the most common taste and odour compounds, is mostly produced by water bloom forming Microcystis. Using the agar-plate method, bacterial strains DH16 and DH18 for degrading β-cyclocitral were isolated from the water samples in the Microcystis dominated water blooms. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that these strains belonged to genera Acidovorax and Acinetobacter respectively. The strain Novosphingobiums sp.THN1, a reported microcystin degraded strain, was also shown to have the ability to de-grade β-cyclocitral. The experiment using β-cyclocitral as the sole carbon source demonstrated that strains DH18 and THN1were able to decomposeβ-cyclocitral efficiently, and these bacterial strains could provide good model organisms for the studying the biodegradation ofβ-cyclocitral.

关 键 词：微囊藻 β-环柠檬醛 食酸菌属 不动杆菌属 新鞘氨醇杆菌属 生物降解 

分 类 号：X172[环境科学与工程—环境科学]