The impact of beta-elemene on beta-tubulin of human hepatoma hepg2 cells  

β-榄香烯注射液对人肝癌HepG-2细胞微管蛋白β的影响(英文)

作　　者：Yuqiu Mao Liying Ban Jielin Zhang Li Hou Xiaonan Cui 

机构地区：[1]Department of Oncology,The First Affiliated Hospital of Dalian Medical University [2]Department of Pathology,Dalian Medical University

出　　处：《The Chinese-German Journal of Clinical Oncology》2014年第2期69-74,共6页中德临床肿瘤学杂志（英文版）

基　　金：Supported by grants from the National Natural Science Foundation of China(No.81173615);the Scientific Research Foundation for the Returned Overseas Chinese Scholars and State Education Ministry;the Specialized Research Fund for the Doctoral Program of Higher Education(No.20102105120002)

摘　　要：Objective: The aim of this study was to investigate the impact of beta-elemene injection on the growth and beta-tubulin of human hepatocarcinoma HepG2 cells. Methods: Cell proliferation was assessed by MTT assay. Cell cycle distribution was detected by flow cytometry(FCM). The mRNA expression of beta-tubulin was measured by RT-PCR. Western blot analysis was used to determine protein expression of beta-tubulin and the polymerization of beta-tubulin. Results: Beta-elemene injection inhibited HepG2 cells proliferation in a dose- and time-dependent manner; FCM analysis indicated beta-elemene injection induced cell cycle arrested at S phase. RT-PCR and western-blot analysis showed that beta-elemene injection down-regulated beta-tubulin expression at both mRNA and protein levels, presenting a dose-dependent manner. Moreover, beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner. Conclusion: Beta-elemene injection can inhibit the proliferation of hepatoma HepG2 cells, the mechanism might be partly related to the down-regulation of beta-tubulin and inhibition of microtubular polymerization.

关 键 词：beta-elemene injection BETA-TUBULIN hepatocellular carcinoma MICROTUBULES tubulin polymerization 

分 类 号：R735.7[医药卫生—肿瘤]