Effects of the anti-tumor composition ofthe acetoacetate extract of vitex negundoseed on the growth of human cervical carcinomaxenografts in nude mice  

黄荆子抗肿瘤活性成分对宫颈癌裸鼠移植瘤生长的影响(英文)

作　　者：Yanlin Cai Zhongdong Chen Aiqiong Tang Bin Jiang Zhaohua Fan Jun Bai 

机构地区：[1]Deparetment of Gynecolgy,The First Affiliated Hospital of University of South China [2]Deparetment of Gynecolgy,Hunan Provincial Maternal and Child Health Hospital [3]Department of Plastic Surgery,The Second Affiliated Hospital of University of South China [4]Deparetment of Obstetrics and Gynecolgy,Hangzhou Red Cross Hospital

出　　处：《The Chinese-German Journal of Clinical Oncology》2014年第3期105-109,共5页中德临床肿瘤学杂志（英文版）

基　　金：Supported by a grant from the Hengyang Municipal Science and Technology Programme(No.2011KJ36)

摘　　要：Objective： The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed （EVn-50） on the growth of human cervical carcinoma HeLa cells xenografts in nude mica and its possible molecular mechanism. Methods： Models of human cervical cancer HeLa cells xenografts transplanted subcuta- neously in nude mice were established and randomly divided into 7 groups （each group including 5 nude mice）： saline group, Taxol group, EVn-50 group, comp-6 group, comp-7 group, comp-8 group and comp-10 group. The volume and weight of Xe- nograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels ofLDH, ALT, Cr and WBC counts were examined and compared. The apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM. The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blot- ting. Results： EVn-50 and its fractionated extracts could significantly suppress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner, yet had no significant effect on the weight of nude mice, the serum levels of LDH, ALT, Cr and WBC were counted. When the xenografts were treated with EVn-50 and its fractionated extracts for 16 days, the apoptotic rate of xenografts cells were significantly increased, and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased. Conclusion： EVn-50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice, which may be related to its pro- motion on xenografts cells apoptosis through down-regulation of Bcl-2 expressionPand activation of P53 expression.

关 键 词：cervical cancer EVn-50 XENOGRAFTS apoptosis 

分 类 号：R737.33[医药卫生—肿瘤]