还原法羊毛角蛋白的提取及对比研究  

The Extraction and Comparison of Wool Keratin Based on Different Reduction Solution Systems

作　　者：王洁[1] 刘建勇[1,2] 王烁[1] 刘坤[1] 

机构地区：[1]天津工业大学纺织学院,天津300387 [2]天津工业大学先进纺织复合材料教育部重点实验室,天津300387

出　　处：《材料导报》2014年第4期46-50,共5页Materials Reports

基　　金：国家科技支撑计划课题(2014BAE01B02)

摘　　要：对5种还原法溶解体系提取羊毛角蛋白的溶解率及大分子的空间结构等做了比较测试和分析。其中,TCEP/亚硫酸氢钠溶解体系溶解时间合理,且溶解率和质量分数高。FTIR测试结果表明,所提取角蛋白的α-螺旋结构和β-折叠结构的含量较原毛都有所下降,而无规卷曲的含量增加明显,但变化程度不一。DSC测试结果表明,提取的角蛋白的α-结晶的熔融峰温各不相同,说明溶解改变了角蛋白大分子的结晶情况,且工艺不同,结晶情况改变程度也不同。The dissolution rate and structure of keratin that were extracted by five kinds of reduction dissolve system were compared. The results shows that dissolved time of TCEP （tris （2-carhoxyethyl） phosphine） / sodium bi- sulfite solution system is reasonable, and the dissolution rate, mass fraction are high. From the Fourier transform in- frared（FTIR） analysis, compared with raw wool, the content of α-helix structure and β-sheet structure of the extrac- ted keratin have fallen, and the content of of random coil increased significantly. However, the degree of change was not the same. From Differential scanning calorimetry （DSC） analysis, the melting temperature of the extraction of keratin are not identical. It shows that the dissolution process changed the macromolecular crystallization of the kera- tin, and because the process is different, the changed degree of the macromolecular crystallization of keratin are diffe- rent.

关 键 词：羊毛 角蛋白 还原法 工艺比较 

分 类 号：TQ340.61[化学工程—化纤工业]