响应面法优化小麦麸皮内源性β–木糖苷酶提取条件  

Study on optimization of extraction conditions of β-xylosidase in wheat bran based on response surface methodology

作　　者：苏东民[1,2] 白瑞[1] 苏东海[3] 黄振华[1] 

机构地区：[1]河南工业大学,河南郑州450001 [2]郑州铁路职业技术学院,河南郑州450052 [3]北京电子科技职业学院,北京100029

出　　处：《粮食与油脂》2014年第3期14-17,共4页Cereals & Oils

基　　金：国家自然科学基金项目(31071597);北京市属高等学校人才强教计划资助项目(PHR201107151)

摘　　要：为提高小麦麸皮内源性β–木糖苷酶提取得率,该文采用响应面法对其提取条件进行优化,在单因素实验基础上,选择提取液pH、料液比、提取时间为自变量,以β–木糖苷酶的酶提取量为响应值,根据Box–Benhnken Design法设计响应面试验,进行显著性和交互作用分析,确定酶的最佳提取条件并进行验证,结果表明:提取液pH 4.93、料液比1∶9.85 g/mL、提取时间36.85 min为最佳提取条件,酶提取量为84.19 mU/g,与响应面拟合优化预测值非常接近。In order to increase the yield of endogenousβ-xylosidase from wheat bran,this article optimized the extraction conditions ofβ-xylosidase by use of response surface methodology. Based on the single factor experiments,it designed the response surface experiments according to Box-Benhnken Design method by taking extraction pH,material-to-water ratio,extraction time as independent variables and the yield of endogenousβ-xylosidase as response value,carried out significance and interaction analysis to determine the optimum extraction conditions,and verified the results. The results showed that the yield of endogenousβ-xylosidase could reach 84.19 mU/g under the optimum extraction conditions of extraction pH 4.93,material-to-water ratio 1∶9.85 g/mL,extraction time 36.85 min,and the result was very close to the predicted value optimized by response surface fitting.

关 键 词：小麦麸皮 内源性β-木糖苷酶 提取条件 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]