超高效液相色谱法测定藻油中的DPA和DHA  

Rapid detection of docosapentenoic acid and docosahexaenoic acid in algae oil by ultra performance liquid chromatography

作　　者：晋文慧[1] 陈伟珠[1] 洪专[1] 张怡评[1] 方华[1] 谢全灵[1] 

机构地区：[1]国家海洋局第三海洋研究所,国家海洋局海洋生物资源综合利用工程技术研究中心,福建厦门361005

出　　处：《食品工业科技》2014年第9期278-281,共4页Science and Technology of Food Industry

基　　金：国家海洋局第三海洋研究所基本业务费专项资金资助项目(海三科2012004);海洋公益性行业科研专项经费项目(200905022);厦门市重大科技创新平台项目(3502Z20111001)

摘　　要：采用超高效液相色谱（UPLC）方法快速分析测定藻油中DPA甲酯和DHA甲酯的含量，考察了流动相、流速及柱温等条件对藻油中DPA-ME和DHA-ME分离的影响，确定了最佳色谱条件：等度洗脱，流动相为乙腈-水（80：20．V／V），流速0．5mL/min，检测波长205nm。该方法下，二十二碳五烯酸甲酯（DPA—ME）和二十二碳六烯酸甲酯（DHA-ME）分别在10．2-510．0、11．1-554．0μg／mL范围内浓度和峰面积呈现良好的线性关系，相关系数r均为0．9999；平均回收率分剐为99．1％（RSD=1.3％）和100．6％（RSD=1.4％）。本方法快速、灵敏、重复性好，适合于藻油中DPA和DHA含量的检测。In order to determine the content of docosapentenoic acid （DPA） and docosahexaenoic acid （DHA） in algae oil quickly and efficiently, a rapid ultra - high pressure liquid chromatography （UPLC） method was developed.The influence of mobile phase,flow rate on the separation of DPA-ME and DHA-ME in algae oil were comprehensively studied.The optimal separation conditions were as follows： a mobile phase consisting of acetonitrile and water（80：20,V/V）with a isocratic elution profile, UV detection wavelength at 205nm,0.SmL/min of flow rate for mobile phase.On the above conditions, good linearity between response （peak area）and concentration was found over a concentration range of 10.2-510.Oμg/mL for DPA-ME, and l l.l-554.0μg/mL for DHA-ME respectively.And the correlation coefficient of the calibration curve of DPA-ME and DHA-ME was 0.9999.The average recovery rates of DPA-ME and DHA-ME were 99.1% （ RSD = 1.3% ）and 100.6% （ RSD = 1.4% ） respectively.This method was high-speed, sensitive and with a good reproducibility.it was appropriate for fast detection of DPA and DHA in algae oil.

关 键 词：超高效液相色谱(UPLC) 二十二碳五烯酸甲酯(DPA—ME) 二十二碳六烯酸甲酯(DHA—ME) 藻油 

分 类 号：TS207.3[轻工技术与工程—食品科学]