高效毛细管电泳同时测定大枣中环磷酸腺苷、芹菜素及槲皮素  

Simultaneous determination of cyclic adenosine monophosphate and apigenin and quercetin in Fructus jujuba by HPCE

作　　者：高瑞斌[1] 杨艳[3] 董树清[2] 倪京满[3] 郭玫[1] 赵亮[2] 

机构地区：[1]甘肃中医学院,药学系,甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000 [2]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室,甘肃省天然药物重点实验室,甘肃兰州730000 [3]兰州大学,药学院,甘肃兰州730000

出　　处：《食品工业科技》2014年第9期282-285,293,共5页Science and Technology of Food Industry

基　　金：中科院科技支新项目(XBXJ-2011-021);兰州市科技专项计划(2010-1-156)

摘　　要：建立高效毛细管电泳（CE）同时测定大枣中环磷酸腺苷、芹菜素及槲皮素的方法。研究了运行缓冲液的浓度、pH、添加剂B-环糊精浓度、检测波长及分离电压对分离结果的影响。在最佳分离测定条件下，三个化学成分得到快速分离检测。环磷酸腺苷、芹菜素及槲皮素分别在16．5-529．4、5．5-176．4、8．8-282．4mg／L范围内线性关系良好，r值分别为0．9998、0．9998和0．9997，加样回收率分别为97．31％、95．62％和94．88％，保留时间RSD值分别为6．7％、5．1％和5．3％，峰面积的RSD值分别为4．5％、5．8％和4．2％。结果表明本方法简便、快速、重现性好，适用于含有环磷酸腺苷、芹菜素及槲皮素类药物成分的检测。High performance capillary electrophoresis（CE） was developed for the determination of cyclic adenosine phosphate,apigenin and quercetin in jujube.The effects of several factors such as concentration of running buffer and the pH,concentration of the additive 15-cyclodextrin,the detection wavelength and the separation voltage for separating result were investigated.In optimum condition, three groups chemical component had been achieved separation detection.A good linearity between peak area ratio of the common peak to the concentration was found in the range of 16.5-529.4mg/L for cyclic adenosine phosphate,5.5- 176.4mg/L for apigenin and 8.8-282.4mg/L for quercetin,and correlation coefficients （r）were 0.9998,0.9998,0.999？, respectively.The average recoveries were 92.31% ,95.62% and 94.88% for cyclic adenosine phosphate, apigenin and quercetin, respectively.The retention time of relative standard deviation was 6.7% ,5.1% and 5.3% and the peak area of relative standard deviation was 4.5% ,5.8% and 4.2%.The results showed that the method was simple, rapid and with satisfactory recoveries and good reproducibilities.lt can be used to detection of elements of cyclic adenosine phosphate, apigenin and quercetin.

关 键 词：大枣 高效毛细管 电泳环磷酸腺苷 芹菜素槲皮素 

分 类 号：TS207.3[轻工技术与工程—食品科学]