1株生防芽孢杆菌的鉴定及其抑菌活性的初步研究  

Identification of a Bacillus amyloliquefaciens Strain and Preliminary Research on Its Antifungal Activity

作　　者：赵辉[1] 鲁传涛[1] 刘红彦[1] 刘玉霞[1] 倪云霞[1] 王鹏涛[1] 

机构地区：[1]河南省农业科学院植物保护研究所/农业部华北南部农作物有害生物综合治理重点实验室/河南省农作物病虫害防治重点实验室,河南郑州450002

出　　处：《河南农业科学》2014年第4期75-79,共5页Journal of Henan Agricultural Sciences

基　　金：河南省基础研究计划项目(122300410051);农业部现代农业产业技术体系专项(CARS-15-1-05)

摘　　要：从芝麻连作田中分离筛选到1株具有拮抗作用的细菌菌株B10-26,为明确其对植物病原真菌的抑菌效果及其分类地位,采用纸碟法及含毒介质法测定了其对部分植物病原真菌的抑菌作用,并通过形态学、生理生化特征以及分子鉴定方法对菌株B10-26的分类地位进行了确定。结果表明,生防菌B10-26菌体和活性物质对菜豆壳球孢(Macrophomina phaseolina)、尖孢镰孢菌(Fusarium oxysporum)、多主棒孢霉(Corynespora cassiicola)和芝麻长蠕孢(Helminthosporium sesami)均有较好的抑制作用。对菌株B10-26的16SrDNA序列同源性和系统发育分析,只能将其鉴定到芽孢杆菌属;对菌株B10-26的ITS序列同源性和系统发育分析表明,B10-26的ITS序列与解淀粉芽孢杆菌(Bacillus amyloliquefaciens)的ITS序列位于同一簇群,同源性达100%。结合形态特征、生理生化特性和ITS序列分析鉴定指标,将B10-26菌株鉴定为解淀粉芽孢杆菌。A bacterial strain B10-26 with antifungal effect was isolated [rom the continuous cropping sesame field. To determine its classification status and evaluate its inhibitory effects on sesame pathogenic fungi, antifungal action of B10-26 was determined using plate test and toxic media method. Identification of the strain B10-26 was carried out by means of phenotypic characteristics observation, physiological and biochemical indexes determination and molecular technique. The results showed that the cells and the active substances of this strain both had high inhibitory effects on sesame pathogenic fungi such as Macrophomina phaseolina, Fusarium oxysporurn, Corynespora cassiicola ,Helminthosporium sesami. Analysis of 16S rDNA sequences only identified B10-26 to the genus Bacillus. It was 100~ homologous to B. amyloliquefaciens by analysis of ITS sequences. The strain B10-26 was confirmed as a strain of B. amyloliquefaciens by combination of phenotypic characteristics, physiological-biochemical properties and phylogenetic analysis result of ITS sequences.

关 键 词：芝麻 解淀粉芽孢杆菌 鉴定 抑菌活性 生物防治 

分 类 号：S435.653[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]