Drosha蛋白在胰腺癌组织中的表达及其临床意义  

Expression of Drosha and its clinicopathological significance in pancreatic cancer tissues

作　　者：曾宗跃[1] 贾微[1] 杨欣[1] 张海龙[1] 柳满然[1] 

机构地区：[1]重庆医科大学临床检验诊断学教育部重点实验室,重庆400016

出　　处：《激光杂志》2014年第4期56-58,共3页Laser Journal

基　　金：国家自然科学基金资助项目(编号:81172296)

摘　　要：目的:探讨胰腺癌组织中Drosha蛋白表达状况及其临床意义。方法:收集胰腺癌样本85例及对应癌旁组织53例,采用H&E和免疫组化法检测胰腺癌组织标本中Drosha蛋白的表达并分析其与临床病理因素之间的关系。采用定量PCR方法检测Drosha基因表达。结果:胰腺癌组织中Drosha的表达阳性率为72.9%(62/85),而相应癌旁Drosha表达阳性率为100%(53/53),两者表达差异有统计学意义(P=0.000),Drosha蛋白在胰腺癌中的阳性强度平均积分值由10.3分降至2.3分。Drosha蛋白的表达与肿瘤的分期(c2=19.2,P=0.0003)、分级(c2=10.8,P=0.013)、淋巴结转移(c2=23.6,P=0.00003)等密切相关。与对应癌旁组织相比,Drosha基因在胰腺癌组织中表达下调(P=0.000)。结论:Drosha蛋白在胰腺癌组织中表达下降,Drosha可能在胰腺癌的发生、发展中具有重要作用。Objective ： To investigate the expression of Drosha and its clinicopathological significance in pancreatic cancer tis- sues. Methods H ＆E staining and immunohistochemical staining technology were used to detect the expression of Drosha, and its cor- relation with clinicopathological characteristics was studied. And the RT-PCR was performed to detect the gene expression level of Drosha. Results The positive rates of Drosha were 76.5%（65/85） in pancreatic cancer tissues and 100% （53/53） in adjacent non-can- cer tissues, and the difference was statistically significant（P 〈0.05）; the expression of Drosha had a significantly positive correlation with the tumor stage（ Chi-Square = 19.2, P = 0.0003）, grade （Chi-Square = 10.8, P =0.013） and lymph node metastasis （Chi-Square = 23.6, P = 0.00003）. The gene expression of Drosha was down-regulated in these paired 53 pancreatic cancer patients compared to those adjacent pancreatic tissues （P =0.000） . Conclusion Drosha is low expressed in pancreatic cancer tissues, and may play impor- tant roles in pancreatic cancer bio~,enesis and progress.

关 键 词：胰腺癌 DROSHA 免疫组化 临床意义 

分 类 号：R735.9[医药卫生—肿瘤]