GDDR对5-FU诱导下胃癌细胞凋亡的影响  

GDDR enhances Caspase-3 activation through 5-FU induced apoptosis in SGC-7901

作　　者：王飞[1] 方诚[1] 赵占伟[1] 王刚[1] 双剑博[1] 华瑾[1] 杜建军[1] 

机构地区：[1]第四军医大学西京消化病医院,陕西西安710032

出　　处：《现代肿瘤医学》2014年第4期780-783,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81071690)

摘　　要：目的:通过5-FU诱导人胃癌细胞系SGC-7901凋亡,研究GDDR在胃癌细胞凋亡中的作用。方法:利用已构建成功的SGC-7901-GDDR和SGC-7901-pcDNA3.1稳定转染细胞系,在5-FU诱导下促进其凋亡发生,流式细胞术和Western blot检测以上两种细胞系中凋亡发生率和Caspase-3表达及活化程度。结果:未加药组中SGC-7901-GDDR和SGC-7901-pcDNA3.1凋亡发生率无统计学意义,且两者均未观察到活化型Caspase-3表达。加药组中SGC-7901-GDDR凋亡发生率显著高于SGC-7901-pcDNA3.1,且活化型Caspase-3表达前者显著高于后者,差异具有统计学意义(P<0.05)。结论:GDDR可通过上调活化型Caspase-3的表达促进SGC-7901细胞凋亡,提示凋亡可能也是GDDR抑癌途径之一,并因此可能在胃肠道肿瘤治疗中发挥作用。Objective：To investigate the role of GDDR in the apoptosis of SGC -7901 cell line. Methods：Previ- ously,we have established the SGC- 7901 -GDDR which expresses GDDR stably and SGC -7901 -pcDNA3.1 as the control cell line. Apoptosis was evaluated using flow cytometry after the two cell lines treated with 5 - FU for 48 hours. The expression of cleaved Caspase - 3 was analyzed by Western blot. Results： After treated with 5 - FU for 48 hours,the apoptosis of SGC -7901 - GDDR was extremely higher compared with SGC -7901 - pcDNA3. 1. This change was reflected by a remarkable increase in the expression levels of cleaved Caspase - 3 （ P 〈 0.05 ）. While in the unteated group, there was no obvious differences observed. Conclusion： The increased expression of cleaved Caspase -3 upregulated by GDDR may play an important role in the apoptosis of gastric cancer,which indicated that GDDR might be an interesting molecular to apply in cancer intervention.

关 键 词：GDDR 细胞凋亡 CASPASE-3 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]