Bcl-XL 反义寡核苷酸对食管癌 EC9706细胞和裸鼠移植瘤组织的诱凋亡作用  

Induced effects of apoptosis on EC9706 cells and xenograft tissue by BclXL antisense oligodeoxynucleotide

作　　者：张岚[1,2] 高冬玲[2] 温洪涛[3] 张蕾[4] 

机构地区：[1]郑州大学基础医学院病理学教研室,郑州450001 [2]郑州大学第一附属医院病理科郑州450052 [3]郑州大学第一附属医院消化内科郑州450052 [4]郑州大学第一附属医院肿瘤科郑州450052

出　　处：《郑州大学学报（医学版）》2014年第2期146-149,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省高校杰出科研人才创新工程基金资助项目2007KYCX005

摘　　要：目的:探讨Bcl-XL反义寡核苷酸(Bcl-XL ASODN)对食管癌EC9706细胞和人食管癌裸鼠移植瘤组织的诱凋亡作用。方法:将合成的Bcl-XL ASODN通过阳离子脂质体介导转染EC9706细胞后,以吖啶橙荧光染色、流式细胞术和TUNEL技术检测ASODN组、细胞对照组、空白对照组和N-ODN组的凋亡率;用TUNEL技术检测ASODN组、N-ODN组和细胞对照组裸鼠移植瘤组织的细胞凋亡率。结果:吖啶橙荧光染色、流式细胞术和TUNEL检测结果显示,ASODN组、N-ODN组、空白对照组和细胞对照组的凋亡率比较,差异均有统计学意义(F=26.853、38.537、28.541,P均<0.001);且ASODN组的凋亡率均高于细胞对照组、空白对照组和N-ODN组(P均<0.05);TUNEL法检测移植瘤组织的细胞凋亡率结果显示,细胞对照组、N-ODN组及ASODN组的凋亡率比较,差异有统计学意义(F=56.375,P<0.001);且ASODN组高于细胞对照组及N-ODN组(P均<0.05)。结论:Bcl-XL ASODN对食管癌EC9706细胞及食管癌裸鼠移植瘤组织均有诱凋亡作用。Aim：To investigate the effects of inducing apoptosis on esophageal cancer EC 9706 cells and xenograft tis-sue of human esophageal cancer in nude mice treated by Bcl-XL antisense oligodeoxynucleotide （ ASODN） .Methods：Bcl-XL ASODN was transfected into EC9706 cells following cationic liposome（ASODN group）.N-ODN group, the cell control group and the blank control group were the control .The apoptosis rate of each cell groups was detected by acridine orange （ AO） fluorescent staining , flow cytometry and TUNEL , respectively .The apoptosis rate in xenograft tissue from nude mice in ASODN group, N-ODN group and the cell control group was detected by TUNEL .Resul ts：The results of AO fluores-cent staining, flow cytometry and TUNEL showed that there were significant differences in apoptosis rates among ASODN group,the cell control group, the blank control group and N-ODN group（F=26.853,38.537, 28.541,P〈0.001）.The ap-optosis rate was higher in ASODN group compared with the cell control ,the blank control and NO-DN groups（P〈0.05）. The apoptosis rates in xenograft tissue detected by TUNEL showed that there were significant differences among ASODN group, the cell control group and N-ODN group（F =563.75, P〈0.001）.The apoptosis rate was higher in ASODN group＆amp;nbsp;compared with the cell control group and N-ODN group（〈0.05）.Conclusion：Bcl-XL ASODN could effectively induce apoptosis of EC9706 cell and xenograft tissue of human esophageal carcinoma .

关 键 词：食管癌 凋亡 Bcl—XL 反义寡核苷酸 EC9706细胞 裸鼠 

分 类 号：R735.1[医药卫生—肿瘤]