An magnetic resonance-based plasma metabonomic investigation on abnormal Savda in different complicated diseases  

作　　者：Batur Mamtimin Mawlanjan Hizbulla Nazuk Kurbantay Li You Xinghai Yan Halmurat Upur 

机构地区：[1]College of Pharmacy,Xinjiang Medical University [2]College of Traditional Chinese Medicine,Xinjiang Medical University

出　　处：《Journal of Traditional Chinese Medicine》2014年第2期166-172,共7页中医杂志（英文版）

基　　金：Supported by China National Natural Science Foundation Program (No.81160459)

摘　　要：OBJECTIVE： To provide existence of abnormal potential evidence for the Savda, we assessed host metabonomic responses and dynamic changes oc- curring in various diseases using 1H nuclear mag- netic resonance （NMR）-based metabonomics. METHODS： Plasma samples taken from patients with complicated diseases with abnormal Savda （n= 140, including 35 cases each of diabetes, asthma, breast cancer, and cervical carcinoma） and from healthy controls （n=35） were analyzed by ~H NMR （600 MHz）, and the spectral profiles were analyzed by multivariate analysis using orthogonal projec- tion to latent structure with discriminant analysis. RESULTS： Supervised modeling of the data provid- ed very good discrimination between patients and healthy controls. Compared with ,the healthy controls, the patient groups with different disease con- ditions displayed similar metabolic changes, characterized by lower creatine, creatinine, lactate, and amino acid levels （including isoleucine, leucine, va- line, alanine, and 1-methylhistidine） and higher lip- id levels （very low-density lipoproteins and unsatu- rated lipids）. Additionally, cancer patients （breast and cervical） showed decreased myo-inositol, a-glucose, and 13-glucose, and increased pyruvate and carnitine in plasma. CONCLUSION： The data indicate that decreased ox- idative defense, liver function abnormalities, amino acid deficiencies, and energy metabolism disorders are common characteristics of complicated diseas- es, which may be related to the formation of abnor- mal Savda.OBJECTIVE: To provide potential evidence for the existence of abnormal Savda, we assessed host metabonomic responses and dynamic changes occurring in various diseases using1H nuclear magnetic resonance(NMR)-based metabonomics.METHODS: Plasma samples taken from patients with complicated diseases with abnormal Savda(n=140, including 35 cases each of diabetes, asthma,breast cancer, and cervical carcinoma) and from healthy controls(n=35) were analyzed by1H NMR(600 MHz), and the spectral profiles were analyzed by multivariate analysis using orthogonal projection to latent structure with discriminant analysis.RESULTS: Supervised modeling of the data provided very good discrimination between patients and healthy controls. Compared with the healthy controls, the patient groups with different disease conditions displayed similar metabolic changes, characterized by lower creatine, creatinine, lactate, and amino acid levels(including isoleucine, leucine, valine, alanine, and 1-methylhistidine) and higher lipid levels(very low-density lipoproteins and unsaturated lipids). Additionally, cancer patients(breast and cervical) showed decreased myo-inositol,α-glucose, and β-glucose, and increased pyruvate and carnitine in plasma.CONCLUSION:The data indicate that decreased oxidative defense, liver function abnormalities, amino acid deficiencies, and energy metabolism disorders are common characteristics of complicated diseases, which may be related to the formation of abnormal Savda.

关 键 词：METABONOMICS Magnetic resonancespectroscopy Abnormal Savda 

分 类 号：R228[医药卫生—中医基础理论]