甘蓝转TA29-Barnase基因植株的花器特征及育性分离的初步研究  

PRELIMINARY STUDY ON FLOWER CHARACTERISTICS AND SEGREGATION OF MALE STERILITY OF TRANSGENIC PLANTS WITH TA29 Barnase IN CABBAGE ( Brassica oleracea var. capitata )

作　　者：朱玉英[1] 龚静[1] 吴晓光[1] 杨红娟[1] 沈革志[2] 王新其[2] 殷丽青[2] 陆桂华[3] 王江[3] 宛新杉[3] 

机构地区：[1]上海市农业科学院园艺研究所,上海201106 [2]上海市农业科学院作物育种栽培研究所,上海201106 [3]中国科学院上海植物生理研究所,上海200032

出　　处：《上海农业学报》2001年第1期79-82,共4页Acta Agriculturae Shanghai

基　　金：上海市科技发展基金项目!(编号 96JC140 0 2 )资助

摘　　要：利用分子生物学方法 ,将控制作物育性的基因 (TA 2 9 Barnase)转化甘蓝生物体 ,培育出了甘蓝雄性不育植株。带有TA 2 9 Barnase基因的雄性不育植株的园艺学性状与未转化植株相同 ,并且其性状在后代中稳定不变 ;不育植株的花朵表现雄蕊完全退化 ,但蜜腺和雌蕊健全 ,能接受外来花粉 ,杂交结实率较高 ;同时 ,雄性不育植株的不育性在后代中出现分离 ,不育株率占 12 .5%～ 85.7% ;此外 。Male sterile cabbage plants were got by transferring TA29 Barnase gene to common cabbage plants. These male sterile transgenic plants with TA29 Barnase gene have the same horticultural characters which can be also inherited steadily by plants in next generation as non transgenic ones. But their flowers bear fully catagenetic stamen lacking pollens, normal fragrant nectaries and strong pistils able to receive pollens from other plants or different cultivars and set seeds. At the meantime, the sterility in transgenic plants can segregate in their posterior generations at sterility rates of 12.5%～85.7%.

关 键 词：甘蓝育种 TA29-BARNASE基因 转基因植株 雄性不育 花器 育性分离 

分 类 号：S635.036[农业科学—蔬菜学]