QuEChERS/高效液相色谱法同时测定水产品中16种多环芳烃  

Simultaneous Determination of 16 Polycyclic Aromatic Hydrocarbons in Aquatic Products by QuEChERS/HPLC

作　　者：万译文 黄向荣[2] 陈湘艺 曾春芳[2] 

机构地区：[1]农业部渔业产品质量监督检验测试中心(长沙),中国长沙410153 [2]湖南省水产科学研究所,中国长沙410153

出　　处：《湖南师范大学自然科学学报》2014年第1期45-50,共6页Journal of Natural Science of Hunan Normal University

基　　金：国家水产品质量安全监测资助项目(2011C028);湖南省养殖业科研项目(201115)

摘　　要：建立了水产品中有16种多环芳烃药物残留量同时测定的高效液相色谱分析方法．样品经乙腈提取，C18与中性氧化铝净化后，采用荧光-紫外检测器串联的方法对多环芳烃进行检测．实验结果表明，16种多环芳烃分离效果好，检出限（LOD）为0．5～5．0μg／kg，相对标准偏差为1．7％～9．2％（n=6），加标回收率达到71．8％～98．8％．该方法具有比较高的重现性和选择性，对于水产品中多环芳烃的残留测定具有很好的应用价值．An analytical method for the determination of 16 polycyclic aromatic hydrocarbons( PAHs) in aquatic products by high performance liquid chromatography was established. The sample was extracted with acetonitril,and the extract was cleaned up with C18and Alumina-N,detected with fluorescence and ultraviolet detector for PAHs. The results showed that the 16 PAHs can be separated efficiently. The detection limits of this method were 0. 5 ~ 5. 0 μg / kg. The relative standard deviations were ranging from 1. 7% ~ 9. 2%,and the recovery rates were from 71. 8% ~ 98. 8%. The method proposed by this paper possesses higher producibility and selectivity,and has promising applications in determining the PAHs in aquatic products.

关 键 词：QUECHERS 高效液相色谱法 多环芳烃 水产品 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]