FA-MNP-MMP-9-ASODN纳米复合物对鼻咽癌的体外抑制研究  

The inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma in vitro

作　　者：刘涛[1] 谢民强[1] 许熠铭[1] 张宏征[1] 张涛[1] 

机构地区：[1]广州南方医科大学珠江医院耳鼻咽喉头颈外科,广州510282

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第9期607-611,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金资助项目(No:81372477;No:81260406);教育部高等学校博士学科点专项科研基金(No:20114433110001)

摘　　要：目的:观察FA-MNP-MMP-9-ASODN纳米复合物对鼻咽癌HNE-1细胞的体外抑制效应。方法:采用RT-PCR、Western-blot方法、MTT方法、流式细胞技术及Matrigel体外侵袭实验观察转染FA-MNP-MMP-9-ASODN 48h后HNE-1细胞MMP-9mRNA及蛋白表达水平和细胞增殖能力、凋亡和侵袭能力的变化。结果:FA-MNP-MMP-9-ASODN组HNE-1细胞MMP-9 mRNA和蛋白的表达水平较对照组及FA-MNP-MMP-9-NSODN无义序列组明显降低。同时FA-MNP-MMP-9-ASODN组HNE-1细胞生长抑制率约为35.66%,增殖活性较对照组和无义序列组明显降低。且HNE-1细胞周期也受到抑制,细胞凋亡率约为12.60%,明显高于对照组和无义序列组。侵袭实验显示FA-MNP-MMP-9-ASODN组穿膜细胞数约为21.00个,明显低于对照组和无义序列组。结论:FA-MNP-MMP-9-ASODN纳米复合物通过抑制MMP-9的表达,可降低鼻咽癌细胞的增殖和侵袭能力,促进凋亡,体外抑制效果良好。Objeclive：To observe the inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma（NPC）HNE-1 cell in vitro. Method：To observe the MMP-9 mRNA and protein expression levels, proliferation ability, cell apoptosis and invasion ability of HNE-1 cell 48 hours after FA-MNP-MMP-9-ASODN transfection by RT-PCR, Western-blot,MTT assay,flow cytometry and Matrigel invasion test. Result： MMP-9 mRNA and protein ex- pression in HNE-1 cell of FA-MNP-MMP-9-ASODN group was significantly decreased compared to control group and nonsense sequence group of FA-MNP-MMP-9-NSODN. At the same time, for the HNE-1 cell in FA-MNP-MMP-9- ASODN group, growth inhibition rate was about 35.66 ;, proliferation activity significantly decreased compared to the control group and the nonsense sequence group, cell cycle was also inhibited, cell apoptosis rate was about 12. 60;, the apoptosis rate was significantly higher than that in the control group and the nonsense sequence group. Invasion assay showed that the transmembrane cells in FA-MNP-MMP-9-ASODN group were about 21.00, significantly lower than that in the control group and the nonsense sequence group. Conclusion： By inhibiting the expression of MMP-9, FA-MNP- MMP-9-ASODN nanocomposites could reduce NPC cell proliferation and invasion ability, and promote apoptosis, it had a good inhibitory effect in vitro.

关 键 词：鼻咽肿瘤 基质金属蛋白酶9 反义寡核苷酸 纳米复合物 

分 类 号：R739.6[医药卫生—肿瘤]