时间分辨荧光免疫层析试纸条在油料饼粕黄曲霉毒素B_1检测中的应用  

Investigation of time- resolved fluoro- immunochromatographic assay strip for quantitative determination of aflatoxin B_1 in oilseed meals

作　　者：李静[1,2,3] 李培武[1,2,4,3,5] 张奇[1,2,4] 丁小霞[1,2,3] 张文[1,2,5] 张兆威[1,2,5] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部生物毒素检测重点实验室,湖北武汉430062 [3]农业部油料产品质量安全风险评估实验室,湖北武汉430062 [4]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [5]农业部油料及制品质量监督检验测试中心,湖北武汉430062

出　　处：《中国油料作物学报》2014年第2期256-262,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家科技支撑计划(2012BAB19B09;2011BAD02D02;2010BAD01B07)

摘　　要：采用黄曲霉毒素时间分辨荧光免疫层析试纸条及配套的时间分辨荧光速测仪,对油料饼粕中黄曲霉毒素B1的快速检测进行了应用研究。该时间分辨荧光免疫分析技术是基于时间分辨荧光免疫层析试纸条和载有Eu（Ⅲ）标记特异性单克隆抗体的样品瓶建立的检测技术。时间分辨荧光速测仪可内置标准曲线,直接输出检测结果。对6种油料饼粕做黄曲霉毒素B1添加回收率实验,回收率在70%~120%之间,批间、批内变异系数〈15%。在实际样品的检测中,时间分辨荧光免疫层析试纸条检测技术与液相色谱-串联质谱法相比,检测结果相对误差〈15%。时间分辨荧光免疫层析试纸条检测技术测定快速、准确,技术稳定、可靠,设备经济、小型,适用于大批量油料饼粕样品的快速检测和风险评估。This research used time- resolved fluoro- immunochromatographic assay strip and assorted time-resolved fluoroimmunoassay（ TRFIA） portable quick tester to detect aflatoxin B1in oilseed meals. The TRFIA technology was based on the sample bottle which contained specific monoclonal antibody marked by Eu（ Ⅲ） and the time- resolved fluoro- immunochromatographic assay strip. The standard curve could be built in the TRFIA portable quick tester and the machine could output the results directly. In the standard addition experiments of six kinds of oilseed meals,the recovery rate of standard addition was 70%- 120%（ CV 15%）. In the determination of practical samples,compared with LC- MS / MS method,the relative error of the time- resolved fluoro- immunochromatographic assay strip method was less than 15%. The results showed that the new method was fast,accu-rate,stable and reliable,and the instrument was cheap and small- sized. Because of these advantages,time- resolved fluoro- immunochromatographic assay strip could be used for aflatoxin B1screening in oilseed meals and risk assessment,and the application prospect would be very broad.

关 键 词：油料饼粕 黄曲霉毒素B1 时间分辨荧光免疫层析试纸条 时间分辨荧光速测仪 定量检测 

分 类 号：S379.7[农业科学—农产品加工]