柱前衍生-HPLC法测定黄酒中氨基酸  

Determination of amino acids in yellow wine by precolumn derivatization coupled with HPLC

作　　者：崔泓[1] 张尧[1] 周东升[1] 

机构地区：[1]十堰市疾病预防控制中心,湖北十堰442000

出　　处：《中国卫生检验杂志》2014年第8期1082-1084,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立柱前衍生-高效液相色谱法测定黄酒中氨基酸的方法。方法样品经酸水解后,用邻苯二甲醛和9-芴甲基氯甲酸酯商品衍生剂进行柱前衍生,高效液相色谱-二极管阵列检测器测定。色谱柱ZORBAX Eclipse AAA4.6 mm×150 mm,5μm氨基酸分析柱,采用梯度洗脱方式,柱温35℃,检测波长:338 nm,进样量20μl。结果在给定的色谱条件下,18种氨基酸在25 min内获得了较好的分离。在0 nmol/ml^1.0 nmol/ml浓度范围内,18种氨基酸峰面积与浓度的线性相关系数为0.9990~0.9997,样品加标回收率在92.9%~103.4%之间,相对标准偏差为0.8%~5.4%。结论该方法操作简单,结果准确,重复性好,可用于黄酒中氨基酸含量的测定。Objective To establish a method for determination of amino acids in yellow wine by precolumn derivatization coupled with HPLC. Methods The samples were hydrolyzed with 0. 10 mol/ L HCl,derivatized with OPA and Fmoc-Cl,then separated on ZORBAX Eclipse amino acid analysis（ AAA） columns（ 4. 6 mm × 150 mm,5 μm） for gradient elution at 35℃, finally determined by HPLC-DAD. The injection volume was 20 μl and the detection wavelength was 338 nm. Results Under the given chromtographic condition,18 kinds of amino acids were separated well in 25 min and linear over the range of 0 nmol/ ml - 1 nmol/ ml（ r = 0. 9990 - 0. 9997） the recovery rates were 92. 9% - 103. 4% and the RSDs were 0. 8%-5. 4%. Conclusion The method is simple,accurate,repeatable,so it can be used to determine the contents of amino acids in yellow wine.

关 键 词：柱前衍生 HPLC 黄酒 氨基酸 

分 类 号：O657.72[理学—分析化学]