姜黄素与HP-β-CD的包合作用机理及理化性质探讨  

Inclusion Mechanism and Physicochemical Property of Curcumin and Hydroxypropyl-beta-cyclodextrin

作　　者：高苏亚[1,2] 王黎[1] 范涛[1] 李华[2] 

机构地区：[1]西安医学院药学院,陕西西安710021 [2]西北大学分析科学研究所,陕西西安710069

出　　处：《化学试剂》2014年第5期389-391,396,共4页Chemical Reagents

基　　金：国家自然科学基金资助项目(20975081);西安医学院博士科研启动基金资助项目(2012DOC12)

摘　　要：采用光度法和Hildebrand-Benesi双倒数方程考察姜黄素与HP-β-CD的包合作用方式和作用机理。升高温度,姜黄素与HP-β-CD的包结常数增大,ΔrGm<0,ΔrHm>0,ΔrSm>0,表明其包结过程是一个自发、吸热的过程,其作用方式主要是一熵驱动下典型的疏水作用。Hildebrand-Benesi双倒数方程线性拟合较好,说明其包合比约为1∶1。据此在不同温度下制备姜黄素-HP-β-CD包合物,采用光谱法表征并验证,测得包合率均>98%,包合物的水溶性和稳定性较姜黄素均有改善。The ultraviolet spectrophotometry and Hildebrand- Benesi double-reciprocal equation were used to investigate theinclusion mechanism and interaction fashion between curcu- rain with hydroxypropyl-beta-cyclodextrin （HP-β-CD）. As the temperature increased, the inclusion constants also increased. Furthermore,the inclusion process was spontaneous endother- mic one from the parameters such as △rGm 〈0,△rHm 〉0 and △rSm 〉 0,respectively. The inclusion interaction between cur- cumin and HP-β-CD was mainly a typical hydrophobic force driven by entropy. A good linearity from Hildebrand-Benesi double-reciprocal equation indicated the bonding ratio was 1：1 approximately. According to the above theory the inclu- sion complexes were prepared at different temperature and were eharaeterized by infrared spectroscopic analysis, respec- tively. In addition, the related physicochemieal properties of the inclusion complex were discussed preliminarily such as inclusion rate, water-solubility and stability, etc. The water- solubility and stability of the inclusion complex were all im- proved with comparison to eurcumin.

关 键 词：姜黄素 HP-β-CD 包合作用 理化性质 

分 类 号：TQ463[化学工程—制药化工] R914.4[医药卫生—药物化学]