高效降解棉酚菌种的筛选及棉饼脱毒参数的研究  

Selection and Study of on High Effective Microbiology for Digesting Freegossypol and Proper Fermentation Processing Data for Detoxification in Cottonseed Cakes

作　　者：杨继良[1] 周大云[1] 杨伟华[1] 朱荷琴[1] 许红霞[1] 夏俊英[1] 冯新爱[1] 项时康[1] 

机构地区：[1]中国农科院棉花研究所,河南安阳455112

出　　处：《棉花学报》2000年第5期225-229,共5页Cotton Science

基　　金：国家"九五"棉粕蛋白工业化生产技术与装备项目的分项目

摘　　要：通过对 6个微生物菌种利用棉酚能力的比较分析 ,筛选出对棉子饼中棉酚具有较强脱毒能力的酵母菌菌株 JM- 3,该菌株可降解棉子饼中80 %以上的游离棉酚。研究表明 :JM- 3的发酵适宜温度为 2 5℃ ,加水量与发酵底物的适宜比值为 0 .8∶ 1 (含水量 45%) ,适宜发酵时间为 36h。该菌种的最大优点是抗杂菌污染能力强 ,生长速度快 ,仅需 36h的发酵 ,就可使棉子饼粕中的棉酚含量降至 0 .0 3%以下 ,蛋白含量提高 3个百分点以上。Comparing and analyzing on the capacity of utilizing free gossypol as carbon resource grow in 6 microbiology races, one high effective detoxification race JM-3 race selected, which can digest 80% free gossypol existing in cottonseed cakes. The study showed that the proper fermentation processing data for this race is as follows: temperature 25 ℃, moisture 45% (water ∶ substrate = 0.8 ∶ 1 ), and 36 hours. The advantages of JM-3 are fast growth and resistant to contamination. It only needs 36 hours to debase free gossypol to less than 0.03%, and increase protein content 3% at least.

关 键 词：棉酚 微生物脱毒 菌种 棉子饼 发酵工艺 饲料 

分 类 号：S562.099[农业科学—作物学]