柔红霉素对人急性早幼粒细胞白血病细胞株HL-60细胞及干扰素调节因子1、8、9表达的影响  

Effect of daunorubicin on the expression of IFN Regulatory Factor 1,-8 and-9 in HL-60 cells

作　　者：刘宏涌[1] 蒋敏[1] 彭荷玲[1] 

机构地区：[1]广西医科大学第一附属医院儿科,南宁市530021

出　　处：《内科》2014年第2期124-127,共4页Internal Medicine

基　　金：广西壮族自治区科技厅科技攻关项目(桂科攻1140003A-30;桂科能0842009-z8)

摘　　要：目的研究柔红霉素(Daunorubicin,DNR)对人急性早幼粒细胞白血病(APL)细胞株HL-60细胞中干扰素调节因子1(IRF1)-mRNA、干扰素调节因子8(IRF8)-mRNA、干扰素调节因子9(IRF9)-mRNA表达的影响。方法将HL-60细胞株设为HL-60组、HL-60+DNR组,同时取3例正常人外周血白细胞为NC组。HL-60组为未加药处理组,HL-60+DNR组为小剂量DNR持续作用HL-60细胞10 d。采用实时荧光定量聚合酶链反应(RT-PCR)法检测IRF1-mRNA、IRF8-mRNA、IRF9-mRNA转录水平,每组实验重复3次。结果与NC组相比,IRF1-mRNA、IRF8-mRNA转录水平在HL-60组显著下调(P<0.05),而HL-60+DNR组显著上调(P<0.05);与NC组相比,IRF9-mRNA转录水平在HL-60+DNR显著上调(P<0.05),在HL-60组则表现为下调,但无统计学差异(P>0.05)。结论 DNR可上调HL-60细胞中的IRF1、IRF8、IRF9表达水平。APL经DNR治疗后,可能通过上调IRF1、IRF8、IRF9的表达诱导白血病细胞的凋亡,促进其成熟,促进病情的缓解。Objective To explore the effect of daunorubicin（DNR）on the expression of IRF1-mRNA,IRF8-mRNA and IRF9-mRNA in acute promyelocytic leukemia cell line of HL-60 cell.Methods HL-60 cell line was set to HL-60 group,HL-60＋DNR group,and the peripheral white blood cells separated from the blood of normal person served as NC group.The HL-60＋DNR group was exposed to small doses of DNR every two day for 10 days,while the HL-60 group received no treatment.IRF1-mRNA,IRF8-mRNA and IRF9-mRNA were detected by real-time reverse transcription polymerase chain reaction（RT-PCR）.The analysis for each group was performed for three times.Results The expression of IRF1-mRNA and IRF8-mRNA in the HL-60 group were markedly down-regulated compared to the NC group（P〈0.05）,but were markedly up-regulated in the HL-60＋DNR group（P〈0.05）;compared to the NC group,the expres-sion of IRF9-mRNA in the HL-60＋DNR group was markedly up-regulated （P〈0.05）,and down-regulated in the HL-60 group,but there was no significant difference between the two groups （P〉0.05）.Conclusions DNR can up-reg-ulate the expression of IRF1、IRF8、IRF9 in HL-60 cell line.DNR may induce apoptosis and promote maturation on leuke-mia cells and promote remission in APL patients.

关 键 词：急性早幼粒细胞白血病 HL-60 细胞 柔红霉素 IRF1 IRF8 IRF9 细胞凋亡 INTERFERON REGULATORY FACTOR 1 Interferon REGULATORY FACTOR 8 Interferon REGULATORY FACTOR 9 

分 类 号：R733.7[医药卫生—肿瘤]