2种豆象线粒体COX1和12S rRNA基因序列的克隆分析  

Cloning and Sequence Analysis on COX1 and 12S rRNA Genes of Two Seed Beetle Species

作　　者：吴培福[1] 佟友贵 王琳 潘涌智[4] 熊忠平[4] 窦报坤[4] 

机构地区：[1]西南林业大学生命科学学院,云南昆明650224 [2]昆明市东川区森防站,云南昆明654100 [3]云南省林业职业技术学院,云南昆明650224 [4]西南林业大学林学院,云南昆明650224

出　　处：《西南林业大学学报（自然科学）》2014年第2期44-48,共5页Journal of Southwest Forestry University：Natural Sciences

基　　金：东川区干热河谷主要树种三种害虫发生规律和防治技术研究项目资助;云南省重点学科建设项目(XKZ200905)资助

摘　　要：分别提取银合欢豆象和合欢豆象的基因组,对其COX1和12S rRNA基因进行测序分析。结果表明:2种豆象的COX1和12S rRNA基因A、T含量较丰富;碱基C和T间有最高的转换率;偏向使用以A或T结尾的密码子;银合欢豆象与合欢豆象进化距离较远,与A.oblongoguttatus进化距离最近,合欢豆象与多型豆象属聚在一个大的分支上。The genome DNA was extracted respectively from Acanthoscelides macrophthalmus and Bruchidius ter-renus,and the COX1 and 12S rRNA genes were sequenced and analyzed to establish the basis for the further molecu-lar study.The results showed that COX1 and 12S rRNA of the two beetle species presented higher content of A＋T, nucleotide C and T showed higher conversion rate.The two genes had codon usage bias as using codons with the third base A or T.It was showed that Acanthoscelides macrophthalmus shared a longer distance with Bruchidius terrenus, but a shorter distance with Acanthoscelides oblongoguttatus.While,Bruchidius terrenus would be grouped into the same branch of the genus Bruchidius.However,the more accurate phylogenetic roles of Acanthoscelides macrophthal-mus and Bruchidius terrenus need to be further studied due to limited nucleotide data in GenBank.

关 键 词：银合欢豆象 合欢豆象 COX1 12S RRNA 测序分析 

分 类 号：S718.7[农业科学—林学]