人脐带间充质干细胞联合番茄红素对衰老小鼠免疫功能的影响  

Effects of human umbilical cord mesenchymal stem cells combined with lycopene on immune function of D-galactose induced aging mice

作　　者：刘佳[1] 王意忠[1] 崔晓兰[2] 时瀚[1] 李倩倩[1] 王琳琳[1] 

机构地区：[1]北京大学航天临床医学院血液内分泌科,北京市100049 [2]中国中医科学院中药所药理室,北京市100700

出　　处：《中国组织工程研究》2014年第14期2206-2212,共7页Chinese Journal of Tissue Engineering Research

基　　金：北京大学航天临床医学院科研基金项目(201209)~~

摘　　要：背景:人脐带间充质干细胞、番茄红素均有延缓衰老的作用,而二者联合应用对衰老机体免疫功能影响的研究却未见报道。目的:分析人脐带间充质干细胞联合番茄红素对D-半乳糖诱导衰老小鼠免疫功能的影响。方法:ICR小鼠雌雄各半,随机分为正常对照组、模型组、番茄红素组、干细胞组及干细胞联合番茄红素组。模型组与各治疗组连续8周颈背部皮下注射D-半乳糖溶液,同时进行相应的治疗干预。造模结束后检测各组小鼠血液衰老相关T淋巴细胞百分比、血清白细胞介素2、白细胞介素4水平并观察脾脏、胸腺组织结构。结果与结论:3个治疗组均能提高衰老小鼠血液CD3+、CD8+CD28+T细胞百分比及血清白细胞介素2、白细胞介素4水平(P<0.01);与模型组相比,联合组初始性CD8+T细胞百分比升高,记忆性CD8+T细胞百分比降低(P<0.05),干细胞及番茄红素组初始性及记忆性CD8+T细胞百分比变化不显著(P>0.05);各组初始性及记忆性CD4+T细胞百分比变化均不显著(P>0.05);胸腺、脾脏镜下观察发现:模型组胸腺小体缩小,脾脏淋巴小结明显增多,髓质淤血;正常对照组与3个治疗组胸腺、脾脏皮质髓质均未见明显病变。结果表明人脐带间充质干细胞、番茄红素均可以不同程度改善衰老小鼠免疫功能,且联合治疗效果更优。BACKGROUND： Both human umbilical cord mesenchymal stem cells and lycopene have the anti-aging actions. But the research about their combination effects on immune function of aging body has not been reported. OBJECTIVE： To investigate the effects of human umbilical cord mesenchymal stem cells combined with lycopene on immune function of D-galactose induced aging mice METHODS： ICR mice, female and male in half, were randomly divided into five groups： normal control group, model group, lycopene group, stem cell group, stem cell combined with lycopene group （combined group）. Model group and treatment groups were injected subcutaneously with D-galactose at the neck for 8 weeks. Age-related percentages of T cell subsets in the blood, contents of interleukin-2 and interleukin-4 in the serum were detected, and structure changes of spleen and thymus were observed. RESULTS AND CONCLUSION： In three treatment groups, the percentages of CD3＋, CD8＋CD28＋ T cells in the blood and contents of interleukin-2 and interleukin-4 in the serum were all improved （P 〈 0.01 ）. In the combined group, naive CD8＋ T cells percentage increased, memory CD8＋ T cells percentage decreased （P 〈 0.05）, however in the stem cell and lycopene groups, nafve and memory CD8＋ T cell percentages did not change significantly （P 〉 0.05）. Nafve and memory CD4＋ T cell percentages between groups did not change significantly （P 〉 0.05）. Microscopic observations of the thymus and spleen showed that in the model group, thymic corpuscles shrunk, splenic nodules significantly increased, splenic medulla was blooded; while no obvious cortical and medullary lesions were observed in the normal control group and three treatment groups. The results showed that both human umbilical cord mesenchymal stem cells and lycopene can ameliorate the immune function of aging model mice, but the effect of combined treatment is better.

关 键 词：脐带间充质干细胞 小鼠免疫功能 番茄红素 延缓衰老 CD8+T细胞 血清白细胞介素2 淋巴细胞百分比 白细胞介素4 

分 类 号：R394.2[医药卫生—医学遗传学]