基于RNA-Seq技术的毕赤酵母基因组重注释及新型强启动子的发现与序列分析  

Genome Re-annotation,Novel Strong Promoter Discovery and Sequence Analysis of Pichia Pastoris Based on RNA-Seq

作　　者：亓飞[1] 蔡孟浩[1] 周祥山[1] 张元兴[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2014年第2期167-175,共9页Journal of East China University of Science and Technology

基　　金：上海市重点学科建设项目(B505)

摘　　要：使用RNA-Seq技术,对毕赤酵母进行了转录组测序。基于测序结果,对现有毕赤酵母基因组进行了重注释,修正了基因组序列中的错误位点861处,发现了新转录本249个及可变剪切现象83个,并更正了553个转录本的错误注释。经过表达谱分析,在毕赤酵母中发现了2个新型强启动子,分别命名为P437和P1431,其驱动的转录本的最高转录水平分别为AOX1基因的1.78倍和3.40倍。序列分析显示,P437和P1431序列中存在着多个酵母转录因子的结合位点。The transcriptome of Pichia pastoris was sequenced using RNA-Seq technique.Based on the sequencing data,the genome of Pichia pastoris was re-annotated,including 861revised positions of the genome sequence,553re-annotated transcripts,249newly discovered transcripts and 83alternative splices.In the analysis of gene expression profiling,2novel strong promoters,named P437 and P1431 respectively,were discovered.The highest transcription levels of the transcripts directed by these promoters are,respectively,1.78times and 3.40times of that of AOX1 gene.Several binding sites of transcription factors were revealed in P437and P1431by sequence analysis.

关 键 词：毕赤酵母 RNA-SEQ 基因组重注释 启动子 

分 类 号：Q933[生物学—微生物学]