固相萃取-气相色谱串联质谱法测定葡萄酒中75种农药残留量  

Multiresidue analysis of seventy-five pesticides in grape wine by GC-MS/MS with SPE pretreatment

作　　者：吴凤琪[1] 沈金灿[1] 吴卫东[1] 蓝芳[1] 

机构地区：[1]深圳出入境检验检疫局食品检验检疫技术中心,深圳市食品安全检测技术研发实验室,深圳518045

出　　处：《食品科技》2014年第5期268-274,共7页Food Science and Technology

基　　金：国家质检总局科技计划项目(2012IK188)

摘　　要：目的：建立葡萄酒中75种农药残留的气相色谱-串联质谱测定方法。方法：样品经二氯甲烷提取，经过CarbonNH2Cartridger复合固相萃取柱净化处理后，采用GC—MS／MS在选择反应监测离子（SRM）@模式下进行质谱定性与内标法定量。结果：在50．0-500．0μg／L范围内，75种农药的回收率在60．0％-110．0％，相对标准偏差（RSD）均在15％以内。结论：所建立的方法简便快捷，可满足检验检疫实际需求。Objective： To established a method for determination of 75 pesticides residues in grape wine by gas chromatography tandem mass spectrometry. Methods： After sample preparation, the sample were extracted with dichloromethane, clean-up was carried out with Carbon-NH2 Cartridges solid phase extraction. The solution was determined and confirmed by GC-MS/MS, quantitative analysis by internal standard method. Results： Within the range of 50.0-500.0 μg/L spiked level, the mean recoveries ranged from 60.0% to 110.0%, with the relative standard deviations （RSDs） under 15%. Conclusion： The proposed method simple and efficient, and can meet the practical needs of inspection and quarantine.

关 键 词：葡萄酒 多种农药残留 GC—MSMS 

分 类 号：TS261.7[轻工技术与工程—发酵工程]