橙皮苷酶高产菌株的选育及其酶学性质的研究  

Screening of Strains Producing Hesperidinase and Studying on Its Characterization

作　　者：申丽静[1] 汪钊[2] 章银军[2] 沈雪亮[2] 

机构地区：[1]嘉兴职业技术学院生物与环境分院,浙江嘉兴314036 [2]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《食品研究与开发》2014年第5期109-112,共4页Food Research and Development

基　　金：浙江省自然科学基金资助项目(编号y304087)

摘　　要：从土样和实验室保藏菌株中筛选得到一株橙皮苷酶的高产菌株Aspergillus niger WZ1，通过紫外诱变和硫酸二乙酯诱变，其酶活力分别提高了74.2%和99.4%，酶活达到3668 U/g固体培养基。利用液质联用法（LC-MS）对酶解产物进行分析鉴定，可知橙皮苷有两种酶解产物，它们分别是橙皮素单糖苷和橙皮素。通过固态发酵制备橙皮苷酶，以橙皮苷作为底物，对该橙皮苷酶的酶学性质进行研究，其最适pH和最适温度分别是5.0和60℃，在pH 3.0～10.0和温度0℃～60℃的环境中，酶的稳定性较好。Aspergillus niger WZ1 was selected from soil and the strains from our laboratory,which could produce hesperidinase with high activity at level of 3 668 U/g solid culture. The original strain was treated by UV and diethyl sulfate, and the enzyme activity was increased 74.2%and 99.4%, respectively. Studying on products was carried out by HPLC-MS. As a result, the products were hesperetin-7-glucoside and hesperetin. The characteristics of hesperidinase were investigated with hesperidin. The optimum pH was 5.0; the optimum temperature was 60℃. The hesperidinase was stable when the pH was 3.0-10.0 and the temperature was 0℃-60℃.

关 键 词：橙皮苷 橙皮苷酶 橙皮素单糖苷 橙皮素 

分 类 号：TQ925[轻工技术与工程—发酵工程]