液质联用法测定淀粉中的马来酸  

Determination of Starch Maleate by LC-MS/MS

作　　者：陈成[1] 施琦贻[1] 

机构地区：[1]上海市食品研究所,上海200235

出　　处：《粮油加工（电子版）》2014年第4期60-62,共3页Machinery for Cereals Oil and Food Processing Main Contents

摘　　要：采用HPLC—ESI—MS／MS联用技术，检测淀粉及其制品中马来酸残留量样品经50％水～50％甲醇提取后，以C18柱为液相分离柱，以甲醇和1％甲酸为流动相，多反应监测模式测定马来酸，其定性定量离子对分别为114．9／71．0。马来酸含量在10～100mg／mL范围内具有良好的线性关系，测定下限（10S／N）为10ng／mL以淀粉为基体，加入3种不同浓度水平的马来酸做加标回收试验，回收率在85％～100％,相对标准偏差（n=6）小于10％该方法操作简单，结果准确．重现性好.Using HPLC-ESI-MS/MS hyphenaled techniques, starch and its products were deteeted residues of maleie aeid. Sample was 50% water and 50% methanol extract in the liquid phase C18 cohmm separalion column with methanol and 1% formie aeid as the mobile phase, multiple reaetion monitoring mode determination nmleie acid, qualitative and quantitative ion pairs were 114.9/71.0. Maleie acid had a good linear relationship within the 10-100ng/mL , detection limit （ IOS/N ） was 10ng/mL. Starch matrix, adding three different concentration levels did maleate spiked reeovery test, recovery reached between 85% to 100% and relative standard deviation （n=6） of less than 10%. This method was simple, accurate, and reproducible.

关 键 词：液质联用 淀粉 马来酸 

分 类 号：TS237[轻工技术与工程—粮食、油脂及植物蛋白工程]